Reference Report

Reference
Citation	White, K.E., Humphrey, D.M., Hirth, F. (2010). The dopaminergic system in the aging brain of Drosophila. Front. Neurosci. 4(): 205. (Export to RIS)
FlyBase ID	FBrf0212667
Publication Type	Research paper
PubMed ID	21165178
PubMed Abstract	Drosophila models of Parkinson's disease are characterized by two principal phenotypes: the specific loss of dopaminergic (DA) neurons in the aging brain and defects in motor behavior. However, an age-related analysis of these baseline parameters in wildtype Drosophila is lacking. Here we analyzed the DA system and motor behavior in aging Drosophila. DA neurons in the adult brain can be grouped into bilateral symmetric clusters, each comprising a stereotypical number of cells. Analysis of TH > mCD8::GFP and cell type-specific MARCM clones revealed that DA neurons show cluster-specific, stereotypical projection patterns with terminal arborization in target regions that represent distinct functional areas of the adult brain. Target areas include the mushroom bodies, involved in memory formation and motivation, and the central complex, involved in the control of motor behavior, indicating that similar to the mammalian brain, DA neurons in the fly brain are involved in the regulation of specific behaviors. Behavioral analysis revealed that Drosophila show an age-related decline in startle-induced locomotion and negative geotaxis. Motion tracking however, revealed that walking activity, and exploration behavior, but not centrophobism increase at late stages of life. Analysis of TH > Dcr2, mCD8::GFP revealed a specific effect of Dcr2 expression on walking activity but not on exploratory or centrophobic behavior, indicating that the siRNA pathway may modulate distinct DA behaviors in Drosophila. Moreover, DA neurons were maintained between early- and late life, as quantified by TH > mCD8::GFP and anti-TH labeling, indicating that adult onset, age-related degeneration of DA neurons does not occur in the aging brain of Drosophila. Taken together, our data establish baseline parameters in Drosophila for the study of Parkinson's disease as well as other disorders affecting DA neurons and movement control.
DOI	10.3389/fnins.2010.00205
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Front. Neurosci.
Title	Frontiers in neuroscience
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ISBN/ISSN	1662-453X 1662-4548
Data from Reference
Alleles (4)
	Dcr-2^Scer\UAS.cDa Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Scer\GAL4^ple.PF Scer\GAL80^αTub84B.PL
Constructs (4)
	P{ple-GAL4.F} P{tubP-GAL80} P{UAS-Dcr-2.D} P{UAS-mCD8::GFP.L}
Genes (8)
	brp Dcr-2 Mmus\Cd8a ple Scer\GAL4 Scer\GAL80 T:Avic\GFP T:Scer\GAL4
Transcripts (1)
	Scer\GAL4^ple.PFRA