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Citation
Venken, K.J., Popodi, E., Holtzman, S.L., Schulze, K.L., Park, S., Carlson, J.W., Hoskins, R.A., Bellen, H.J., Kaufman, T.C. (2010). A molecularly defined duplication set for the X chromosome of Drosophila melanogaster.  Genetics 186(4): 1111--1125.
FlyBase ID
FBrf0212670
Publication Type
Research paper
Abstract
We describe a molecularly defined duplication kit for the X chromosome of Drosophila melanogaster. A set of 408 overlapping P[acman] BAC clones was used to create small duplications (average length 88 kb) covering the 22-Mb sequenced portion of the chromosome. The BAC clones were inserted into an attP docking site on chromosome 3L using ΦC31 integrase, allowing direct comparison of different transgenes. The insertions complement 92% of the essential and viable mutations and deletions tested, demonstrating that almost all Drosophila genes are compact and that the current annotations of the genome are reasonably accurate. Moreover, almost all genes are tolerated at twice the normal dosage. Finally, we more precisely mapped two regions at which duplications cause diplo-lethality in males. This collection comprises the first molecularly defined duplication set to cover a whole chromosome in a multicellular organism. The work presented removes a long-standing barrier to genetic analysis of the Drosophila X chromosome, will greatly facilitate functional assays of X-linked genes in vivo, and provides a model for functional analyses of entire chromosomes in other species.
PubMed ID
PubMed Central ID
PMC2998297 (PMC) (EuropePMC)
Related Publication(s)
Personal communication to FlyBase
Small X duplications for the stock center collection.
Popodi et al., 2010-, Small X duplications for the stock center collection. [FBrf0210621]
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Genetics
    Title
    Genetics
    Publication Year
    1916-
    ISBN/ISSN
    0016-6731
    Data From Reference
    Aberrations (399)
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    Alleles (1)
    Genes (134)
    Datasets (3)
    Insertions (1)