Reference Report

Reference
Citation	Benhra, N., Lallet, S., Cotton, M., Le Bras, S., Dussert, A., Le Borgne, R. (2011). AP-1 Controls the Trafficking of Notch and Sanpodo toward E-Cadherin Junctions in Sensory Organ Precursors. Curr. Biol. 21(1): 87--95. (Export to RIS)
FlyBase ID	FBrf0212697
Publication Type	Research paper
PubMed ID	21194948
PubMed Abstract	In Drosophila melanogaster, external sensory organs develop from a single sensory organ precursor (SOP). The SOP divides asymmetrically to generate daughter cells, whose fates are governed by differential Notch activation. Here we show that the clathrin adaptor AP-1 complex, localized at the trans Golgi network and in recycling endosomes, acts as a negative regulator of Notch signaling. Inactivation of AP-1 causes ligand-dependent activation of Notch, leading to a fate transformation within sensory organs. Loss of AP-1 affects neither cell polarity nor the unequal segregation of the cell fate determinants Numb and Neuralized. Instead, it causes apical accumulation of the Notch activator Sanpodo and stabilization of both Sanpodo and Notch at the interface between SOP daughter cells, where DE-cadherin is localized. Endocytosis-recycling assays reveal that AP-1 acts in recycling endosomes to prevent internalized Spdo from recycling toward adherens junctions. Because AP-1 does not prevent endocytosis and recycling of the Notch ligand Delta, our data indicate that the DE-cadherin junctional domain may act as a launching pad through which endocytosed Notch ligand is trafficked for signaling.
DOI	10.1016/j.cub.2010.12.010
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Curr. Biol.
Title	Current Biology
Publication Year	1991-
ISBN/ISSN	0960-9822
Data from Reference
Aberrations (1)
	Df(3R)Exel6264
Alleles (19)
	AP-1γ^GD1711 AP-1μ^GD14206 AP-1μ^SAE-10 AP-1μ^{Scer\UAS.T:Avic\GFP-YFP.Venus} AP-1μ^SHE-11 AP-2α^40-31 Disc\RFP^{Scer\UAS.P\T.T:Hsap\Golgi} Dl^RevF10 Scer\GAL4^ap-md544 Scer\GAL4^neur-P72 Ser^RX106 spdo^G104 spdo^{L2.Scer\UAS.T:Disc\RFP-mCherry} spdo^Scer\UAS.cOa spdo^{Scer\UAS.T:Disc\RFP-mCherry} spdo^{Δ18.Scer\UAS.T:Disc\RFP-mCherry} T:Avic\GFP^YFP.Venus T:Disc\RFP^mCherry WASp³
Constructs (7)
	P{GD1711} P{GD14206} P{UAS-AP-47.VFP} P{UAS-Cherry-spdo.L2} P{UASp-RFP.Golgi} P{UAS-Spdo.mCherry} P{UAS-Spdo.Δ18.mCherry}
Genes (22)
	AP-1-2β AP-1γ AP-1μ AP-1σ AP-2α AP-2μ ct Disc\RFP Dl elav lqfR N neur numb Rab11 Scer\GAL4 Ser shg spdo Su(H) TfAP-2 WASp
Insertions (2)
	P{GAL4}neur^P72 P{GawB}ap^md544
Natural transposons (1)
	P-element