Reference Report

Reference
Citation	Kondo, S., Perrimon, N. (2011). A Genome-Wide RNAi Screen Identifies Core Components of the G2-M DNA Damage Checkpoint. Sci. Signal. 4(154): rs1. (Export to RIS)
FlyBase ID	FBrf0212700
Publication Type	Research paper
PubMed ID	21205937
PubMed Abstract	The DNA damage checkpoint, the first pathway known to be activated in response to DNA damage, is a mechanism by which the cell cycle is temporarily arrested to allow DNA repair. The checkpoint pathway transmits signals from the sites of DNA damage to the cell cycle machinery through the evolutionarily conserved ATM (ataxia telangiectasia mutated) and ATR (ATM- and Rad3-related) kinase cascades. We conducted a genome-wide RNAi (RNA interference) screen in Drosophila cells to identify previously unknown genes and pathways required for the G₂-M checkpoint induced by DNA double-strand breaks (DSBs). Our large-scale analysis provided a systems-level view of the G₂-M checkpoint and revealed the coordinated actions of particular classes of proteins, which include those involved in DNA repair, DNA replication, cell cycle control, chromatin regulation, and RNA processing. Further, from the screen and in vivo analysis, we identified previously unrecognized roles of two DNA damage response genes, mus101 and mus312. Our results suggest that the DNA replication preinitiation complex, which includes MUS101, and the MUS312-containing nuclease complexes, which are important for DSB repair, also function in the G₂-M checkpoint. Our results provide insight into the diverse mechanisms that link DNA damage and the checkpoint signaling pathway.
DOI	10.1126/scisignal.2001350
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Sci. Signal.
Title	Science signaling
Publication Year
ISBN/ISSN	1937-9145
Data from Reference
Alleles (20)
	Avic\GFP^Scer\UAS.cUa CDC45L^NIG.3658R grp^JF02588 Mcm3^NUG.cUa Mcm7^{dsRNA.Scer\UAS.cKa} mei-9^A1 mei-41^RT1 mms4^ex1 mus81^Nhe mus101^D1 mus101^D2 mus101^K451 mus101^SM mus312^D1 mus312^{TRiP.HMS00192} mus312^Z1973 mus312^Z3997 RecQ4^NIG.7487R Scer\GAL4^ap-md544 slx1^{TRiP.HMS00313}
Constructs (8)
	P{NIG.3658R} P{NIG.7487R} P{NIG.Mcm3.U} P{TRiP.HMS00192} P{TRiP.HMS00313} P{TRiP.JF02588} P{UAS-GFP.U} P{UAS-Mcm7.RNAi}
Genes (74)
	14-3-3ε Ald alien Avic\GFP BRWD3 CDC45L Cdk8 CG9797 CSN1b CSN3 CSN4 CSN5 CSN6 CSN7 CSN8 Cul-4 CycC dgt1 E(bx) eIF4G FANCI gfzf grp His2Av His3 Kap-α3 lin19 lwr MBD-R2 Mcm3 Mcm7 me31B MED4 MED17 mei-9 mei-41 mms4 mre11 mus81 mus101 mus304 mus312 Myt1 nbs Not1 pic pont Pop2 Rbf Rcd1 Rcd5 RecQ4 rept Rga RpA-70 Rpb4 Rpb5 Rpb8 RpII15 RpII18 RpII215 Scer\GAL4 SF2 skpA slx1 Spt5 Su(var)2-10 Tango6 tefu Top2 Topors wah wds yeti
Insertions (2)
	P{}mei-41^RT1 P{GawB}ap^md544
Natural transposons (1)
	P-element