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Citation
Pek, J.W., Kai, T. (2011). A role for vasa in regulating mitotic chromosome condensation in Drosophila.  Curr. Biol. 21(1): 39--44.
FlyBase ID
FBrf0212718
Publication Type
Research paper
Abstract

Vasa (Vas) is a conserved DEAD-box RNA helicase expressed in germline cells that localizes to a characteristic perinuclear structure called nuage. Previous studies have shown that Vas has diverse functions, with roles in regulating mRNA translation, germline differentiation, pole plasm assembly, and piwi-interacting RNA (piRNA)-mediated transposon silencing. Although vas has also been implicated in the regulation of germline proliferation in Drosophila and mice, little is known about whether Vas plays a role during the mitotic cell cycle. Here, we report a translation-independent function of vas in regulating mitotic chromosome condensation in the Drosophila germline. During mitosis, Vas facilitates robust chromosomal localization of the condensin I components Barren (Barr) and CAP-D2. Vas specifically associates with Barr and CAP-D2, but not with CAP-D3 (a condensin II component). The mitotic function of Vas is mediated by the formation of perichromosomal Vas bodies during mitosis, which requires the piRNA pathway components aubergine and spindle-E. Our results suggest that Vas functions during mitosis and may link the piRNA pathway to mitotic chromosome condensation in Drosophila.

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Curr. Biol.
    Title
    Current Biology
    Publication Year
    1991-
    ISBN/ISSN
    0960-9822
    Data From Reference
    Aberrations (1)
    Alleles (13)
    Genes (15)
    Physical Interactions (7)
    Natural transposons (1)
    Experimental Tools (2)
    Transgenic Constructs (4)