Reference Report

Reference
Citation	Abuin, L., Bargeton, B., Ulbrich, M.H., Isacoff, E.Y., Kellenberger, S., Benton, R. (2011). Functional architecture of olfactory ionotropic glutamate receptors. Neuron 69(1): 44--60. (Export to RIS)
FlyBase ID	FBrf0212725
Publication Type	Research paper
PubMed ID	21220098
PubMed Abstract	Ionotropic glutamate receptors (iGluRs) are ligand-gated ion channels that mediate chemical communication between neurons at synapses. A variant iGluR subfamily, the Ionotropic Receptors (IRs), was recently proposed to detect environmental volatile chemicals in olfactory cilia. Here, we elucidate how these peripheral chemosensors have evolved mechanistically from their iGluR ancestors. Using a Drosophila model, we demonstrate that IRs act in combinations of up to three subunits, comprising individual odor-specific receptors and one or two broadly expressed coreceptors. Heteromeric IR complex formation is necessary and sufficient for trafficking to cilia and mediating odor-evoked electrophysiological responses in vivo and in vitro. IRs display heterogeneous ion conduction specificities related to their variable pore sequences, and divergent ligand-binding domains function in odor recognition and cilia localization. Our results provide insights into the conserved and distinct architecture of these olfactory and synaptic ion channels and offer perspectives into the use of IRs as genetically encoded chemical sensors. VIDEO ABSTRACT:
DOI	10.1016/j.neuron.2010.11.042
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Neuron
Title	Neuron
Publication Year	1988-
ISBN/ISSN	0896-6273
Data from Reference
Alleles (30)
	Disc\RFP^{mCherry.Scer\UAS.T:Hsap\CALR} Ir8a¹ Ir8a^{D724A.Scer\UAS.T:Hsap\CALR,T:Avic\GFP-EGFP} Ir8a^{R481A.Scer\UAS.T:Hsap\CALR,T:Avic\GFP-EGFP} Ir8a^{R481E.Scer\UAS.T:Hsap\CALR,T:Avic\GFP-EGFP} Ir8a^Scer\UAS.cAa Ir8a^{Scer\UAS.T:Hsap\CALR,T:Avic\GFP-EGFP} Ir8a^{Scer\UAS.T:Hsap\CALR,T:Disc\RFP-mCherry} Ir8a^{T645A.Scer\UAS.T:Hsap\CALR,T:Avic\GFP-EGFP} Ir8a^{ΔATD.Scer\UAS.T:Hsap\CALR,T:Avic\GFP-EGFP} Ir8a^{ΔC.Scer\UAS.T:Hsap\CALR,T:Avic\GFP-EGFP} Ir25a² Ir25a^Scer\UAS.cAa Ir64a^{Scer\UAS.T:Hsap\CALR,T:Avic\GFP-EGFP} Ir76a^Scer\UAS.cBa Ir76b^Scer\UAS.cAa Ir84a^GAL4 Ir84a^{R317A.Scer\UAS.T:Hsap\CALR,T:Avic\GFP-EGFP} Ir84a^Scer\UAS.cBa Ir84a^{Scer\UAS.T:Hsap\CALR,T:Avic\GFP-EGFP} Ir84a^{ΔC.Scer\UAS.T:Hsap\CALR,T:Avic\GFP-EGFP} Ir84a^{ΔN.Scer\UAS.T:Hsap\CALR,T:Avic\GFP-EGFP} Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Scer\GAL4^Ir8a.PA Scer\GAL4^Ir25a.PA Scer\GAL4^Ir84a-GAL4 Scer\GAL4^Or22a.7.717 T:Avic\GFP^EGFP T:Disc\RFP^mCherry αTub84B^{Scer\UAS.P\T.T:Avic\GFP-S65C}
Constructs (25)
	P{GAL4} P{Ir8a-GAL4.A} P{Ir25a-GAL4.A} P{Or22a-GAL4.7.717} P{UAS-Ir8a.attB} P{UAS-Ir8a.CALR.EGFP.attB} P{UAS-Ir8a.D724A.CALR.EGFP.attB} P{UAS-Ir8a.mCherry.CALR.attB} P{UAS-Ir8a.R481A.CALR.EGFP.attB} P{UAS-Ir8a.R481E.CALR.EGFP.attB} P{UAS-Ir8a.T645A.CALR.EGFP.attB} P{UAS-Ir8a.ΔATD.CALR.EGFP.attB} P{UAS-Ir8a.ΔC.CALR.EGFP.attB} P{UAS-Ir25a.attB} P{UAS-Ir64a.attB} P{UAS-Ir76a.B} P{UAS-Ir76b.A} P{UAS-Ir84a.B} P{UAS-Ir84a.CALR.EGFP.attB} P{UAS-Ir84a.R317A.CALR.EGFP.attB} P{UAS-Ir84a.ΔC.CALR.EGFP.attB} P{UAS-Ir84a.ΔN.CALR.EGFP.attB} P{UAS-mCD8::GFP.L} P{UAS-mCherry.CALR.attB} P{UASp-GFPS65C-αTub84B}
Genes (13)
	Disc\RFP Ir8a Ir25a Ir64a Ir75a Ir76a Ir76b Ir84a Mmus\Cd8a Or22a Scer\GAL4 T:Hsap\CALR αTub84B
Natural transposons (1)
	P-element
Transcripts (2)
	Scer\GAL4^Ir8a.PARA Scer\GAL4^Ir84a-GAL4RA