Reference Report

Reference
Citation	Djiane, A., Shimizu, H., Wilkin, M., Mazleyrat, S., Jennings, M.D., Avis, J., Bray, S., Baron, M. (2011). Su(dx) E3 ubiquitin ligase-dependent and -independent functions of Polychaetoid, the Drosophila ZO-1 homologue. J. Cell Biol. 192(1): 189--200. (Export to RIS)
FlyBase ID	FBrf0212737
Publication Type	Research paper
PubMed ID	21200027
PubMed Abstract	Zona occludens (ZO) proteins are molecular scaffolds localized to cell junctions, which regulate epithelial integrity in mammals. Using newly generated null alleles, we demonstrate that polychaetoid (pyd), the unique Drosophila melanogaster ZO homologue, regulates accumulation of adherens junction-localized receptors, such as Notch, although it is dispensable for epithelial polarization. Pyd positively regulates Notch signaling during sensory organ development but acts negatively on Notch to restrict the ovary germline stem cell niche. In both contexts, we identify a core antagonistic interaction between Pyd and the WW domain E3 ubiquitin ligase Su(dx). Pyd binds Su(dx) directly, in part through a noncanonical WW-binding motif. Pyd also restricts epithelial wing cell numbers to control adult wing shape, a function associated with the FERM protein Expanded and independent of Su(dx). As both Su(dx) and Expanded regulate trafficking, we propose that a conserved role of ZO proteins is to coordinate receptor trafficking and signaling with junctional organization.
DOI	10.1083/jcb.201007023
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Cell Biol.
Title	Journal of Cell Biology
Publication Year	1966-
ISBN/ISSN	0021-9525
Data from Reference
Aberrations (2)
	Df(2L)Exel6006 Df(3R)p-XT103
Alleles (33)
	Cdk4³ Cdk4^k06503 ex^e1 N^55e11 Pten^c494 Pten^dj189 pyd^C5 pyd^{dsRNA.Scer\UAS} pyd^ex79 pyd^ex147 pyd^ex180 pyd^NP4400 pyd^{Scer\UAS.T:Avic\GFP} pyd^tam pyd^{ΔEx6.Scer\UAS.T:Avic\GFP} pyd^{ΔEx6ΔPro-Rich.Scer\UAS.T:Avic\GFP} pyd^{ΔGUK.Scer\UAS.T:Avic\GFP} pyd^{ΔPDZ1.Scer\UAS.T:Avic\GFP} pyd^{ΔPDZ2.Scer\UAS.T:Avic\GFP} pyd^{ΔPDZ3.Scer\UAS.T:Avic\GFP} pyd^{ΔPro-Rich.Scer\UAS.T:Avic\GFP} pyd^{ΔSH3.Scer\UAS.T:Avic\GFP} Scer\GAL4^Act5C.PU Scer\GAL4^dpp.PU Scer\GAL4^ptc-559.1 Scer\GAL4^sca-537.4 Su(dx)³² Su(dx)⁵⁶ Su(dx)^Scer\UAS.cCa Su(dx)^sp Su(H)⁸ Tor^ΔP yps^JM2
Constructs (13)
	P{Act5C-GAL4.U} P{dpp-GAL4.U} P{UAS-pyd.FL} P{UAS-pyd.RNAi} P{UAS-pyd.ΔEx6} P{UAS-pyd.ΔEx6ΔPro-Rich} P{UAS-pyd.ΔGUK} P{UAS-pyd.ΔPDZ1} P{UAS-pyd.ΔPDZ2} P{UAS-pyd.ΔPDZ3} P{UAS-pyd.ΔPro-Rich} P{UAS-pyd.ΔSH3} P{UAS-Su(dx).C}
Genes (20)
	aPKC arm Cdk4 dlg1 en ex ft N Pten pyd Scer\GAL4 scrib sens shg shot Su(dx) Su(H) T:Avic\GFP Tor yps
Insertions (3)
	P{GawB}ptc^559.1 P{GawB}pyd^NP4400 P{GawB}sca^537.4
Natural transposons (1)
	P-element