Reference Report

Reference
Citation	Slawson, J.B., Kuklin, E.A., Ejima, A., Mukherjee, K., Ostrovsky, L., Griffith, L.C. (2011). Central Regulation of Locomotor Behavior of Drosophila melanogaster Depends on a CASK Isoform Containing CaMK-Like and L27 Domains. Genetics 187(1): 171--184. (Export to RIS)
FlyBase ID	FBrf0212754
Publication Type	Research paper
PubMed ID	21059886
PubMed Abstract	Genetic causes for disturbances of locomotor behavior can be due to muscle, peripheral neuron, or central nervous system pathologies. The Drosophila melanogaster homolog of human CASK (also known as caki or camguk) is a molecular scaffold that has been postulated to have roles in both locomotion and plasticity. These conclusions are based on studies using overlapping deficiencies that largely eliminate the entire CASK locus, but contain additional chromosomal aberrations as well. More importantly, analysis of the sequenced Drosophila genome suggests the existence of multiple protein variants from the CASK locus, further complicating the interpretation of experiments using deficiency strains. In this study, we generated small deletions within the CASK gene that eliminate gene products containing the CaMK-like and L27 domains (CASK-β), but do not affect transcripts encoding the smaller forms (CASK-α), which are structurally homologous to vertebrate MPP1. These mutants have normal olfactory habituation, but exhibit a striking array of locomotor problems that includes both initiation and motor maintenance defects. Previous studies had suggested that presynaptic release defects at the neuromuscular junction in the multigene deficiency strain were the likely basis of its locomotor phenotype. The locomotor phenotype of the CASK-β mutant, however, cannot be rescued by expression of a CASK-β transgene in motor neurons. Expression in a subset of central neurons that does not include the ellipsoid body, a well-known pre-motor neuropil, provides complete rescue. Full-length CASK-β, while widely expressed in the nervous system, appears to have a unique role within central circuits that control motor output.
DOI	10.1534/genetics.110.123406
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (1)
	Df(3R)Exel6187
Alleles (11)
	CASK^P18 CASK^P29 CASK^P46 CASK^Scer\UAS.cBa CASK^T119 CASK^X-307 CASK^X-313 Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Scer\GAL4^C164 Scer\GAL4^elav-C155 Scer\GAL4^VGlut-OK371
Constructs (2)
	P{UAS-Cmg.B} P{UAS-mCD8::GFP.L}
Genes (3)
	CASK Mmus\Cd8a Scer\GAL4
Insertions (4)
	P{EPgy2}CASK^EY07081 P{GawB}C164 P{GawB}elav^C155 P{GawB}VGlut^OK371