Reference Report

Reference
Citation	Hatan, M., Shinder, V., Israeli, D., Schnorrer, F., Volk, T. (2011). The Drosophila blood brain barrier is maintained by GPCR-dependent dynamic actin structures. J. Cell Biol. 192(2): 307--319. (Export to RIS)
FlyBase ID	FBrf0212807
Publication Type	Research paper
PubMed ID	21242289
PubMed Abstract	The blood brain barrier (BBB) is essential for insulation of the nervous system from the surrounding environment. In Drosophila melanogaster, the BBB is maintained by septate junctions formed between subperineurial glia (SPG) and requires the Moody/G protein-coupled receptor (GPCR) signaling pathway. In this study, we describe novel specialized actin-rich structures (ARSs) that dynamically form along the lateral borders of the SPG cells. ARS formation and association with nonmuscle myosin is regulated by Moody/GPCR signaling and requires myosin activation. Consistently, an overlap between ARS localization, elevated Ca(2+) levels, and myosin light chain phosphorylation is detected. Disruption of the ARS by inhibition of the actin regulator Arp2/3 complex leads to abrogation of the BBB. Our results suggest a mechanism by which the Drosophila BBB is maintained by Moody/GPCR-dependent formation of ARSs, which is supported by myosin activation. The localization of the ARSs close to the septate junctions enables efficient sealing of membrane gaps formed during nerve cord growth.
DOI	10.1083/jcb.201007095
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Cell Biol.
Title	Journal of Cell Biology
Publication Year	1966-
ISBN/ISSN	0021-9525
Data from Reference
Aberrations (1)
	Df(1)moody-Δ17
Alleles (19)
	Arp3^GD12273 Arp3^{Scer\UAS.P\T.T:Avic\GFP} Arpc1^GD14085 Avic\GFP^{GCaMP3.Scer\UAS} Avic\GFP^Nrg-G00305 Avic\GFP^Nrx-IV-PTT Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Moe^{GMA.Scer\UAS.T:Avic\GFP-S65T} moody^{α.Scer\UAS.T:Avic\GFP} moody^{β.Scer\UAS.T:Avic\GFP} Scer\ABP140^{Scer\UAS.T:Avic\GFP} Scer\ABP140^{Scer\UAS.T:Disc\RFP-Ruby} Scer\FLP1^repo.PK Scer\GAL4^moody.SPG scrib^{Scer\UAS.P\T.T:Avic\GFP} sqh^{RLC.T:Avic\GFP-S65T} T:Disc\RFP^Ruby zip^{DN.Scer\UAS.T:Avic\GFP} zip^{FL.Scer\UAS.T:Avic\GFP}
Constructs (16)
	P{GD12273} P{GD14085} P{moody-GAL4.SPG} P{repo-flp1.K} P{sqh-GFP.RLC} P{UAS-GCaMP3.T} P{UAS-GMA} P{UAS-Lifeact-GFP} P{UAS-Lifeact-Ruby} P{UAS-mCD8::GFP.L} P{UAS-moody-α-GFP} P{UAS-moody-β-GFP} P{UASp-Arp3.GFP} P{UASp-scrib.GFP} P{UAS-zip.GFP.DN} P{UAS-zip.GFP.FL}
Genes (15)
	Arp3 Arpc1 Avic\GFP Mmus\Cd8a Moe moody Nrg Nrx-IV Scer\ABP140 Scer\FLP1 Scer\GAL4 scrib sqh T:Avic\GFP zip
Insertions (2)
	P{PTT-GA}Nrg^G00305 P{PTT-un1}Nrx-IV^PTT
Natural transposons (1)
	P-element