Open Close
Reference
Citation
Kim, Y., Paroush, Z., Nairz, K., Hafen, E., JimĂ©nez, G., Shvartsman, S.Y. (2011). Substrate-dependent control of MAPK phosphorylation in vivo.  Mol. Syst. Biol. 7(): 467.
FlyBase ID
FBrf0212870
Publication Type
Research paper
Abstract

Phosphorylation of the mitogen-activated protein kinase (MAPK) is essential for its enzymatic activity and ability to control multiple substrates inside a cell. According to the current models, control of MAPK phosphorylation is independent of its substrates, which are viewed as mere sensors of MAPK activity. Contrary to this modular view of MAPK signaling, our studies in the Drosophila embryo demonstrate that substrates can regulate the level of MAPK phosphorylation in vivo. We demonstrate that a twofold change in the gene dosage of a single substrate can induce a significant change in the phosphorylation level of MAPK and in the conversion of other substrates. Our results support a model where substrates of MAPK counteract its dephosphorylation by phosphatases. Substrate-dependent control of MAPK phosphorylation is a manifestation of a more general retroactive effect that should be intrinsic to all networks with covalent modification cycles.

PubMed ID
PubMed Central ID
PMC3063690 (PMC) (EuropePMC)
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Mol. Syst. Biol.
    Title
    Molecular Systems Biology
    Publication Year
    2005-
    ISBN/ISSN
    1744-4292
    Data From Reference
    Genes (9)