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Citation
Chung, K., Kim, Y., Kanodia, J.S., Gong, E., Shvartsman, S.Y., Lu, H. (2011). A microfluidic array for large-scale ordering and orientation of embryos.  Nat. Methods 8(2): 171--176.
FlyBase ID
FBrf0212871
Publication Type
Research paper
Abstract

Quantitative studies of embryogenesis require the ability to monitor pattern formation and morphogenesis in large numbers of embryos, at multiple time points and in diverse genetic backgrounds. We describe a simple approach that greatly facilitates these tasks for Drosophila melanogaster embryos, one of the most advanced models of developmental genetics. Based on passive hydrodynamics, we developed a microfluidic embryo-trap array that can be used to rapidly order and vertically orient hundreds of embryos. We describe the physical principles of the design and used this platform to quantitatively analyze multiple morphogen gradients in the dorsoventral patterning system. Our approach can also be used for live imaging and, with slight modifications, could be adapted for studies of pattern formation and morphogenesis in other model organisms.

PubMed ID
PubMed Central ID
PMC4100329 (PMC) (EuropePMC)
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Nat. Methods
    Title
    Nature Methods
    Publication Year
    2004-
    ISBN/ISSN
    1548-7091 1548-7105
    Data From Reference
    Genes (5)