Reference Report

Reference
Citation	Guerrero, P.A., Maggert, K.A. (2011). The CCCTC-Binding Factor (CTCF) of Drosophila Contributes to the Regulation of the Ribosomal DNA and Nucleolar Stability. PLoS ONE 6(1): e16401. (Export to RIS)
FlyBase ID	FBrf0212883
Publication Type	Research paper
PubMed ID	21283722
PubMed Abstract	In the repeat array of ribosomal DNA (rDNA), only about half of the genes are actively transcribed while the others are silenced. In arthropods, transposable elements interrupt a subset of genes, often inactivating transcription of those genes. Little is known about the establishment or separation of juxtaposed active and inactive chromatin domains, or preferential inactivation of transposable element interrupted genes, despite identity in promoter sequences. CTCF is a sequence-specific DNA binding protein which is thought to act as a transcriptional repressor, block enhancer-promoter communication, and delimit juxtaposed domains of active and inactive chromatin; one or more of these activities might contribute to the regulation of this repeated gene cluster. In support of this hypothesis, we show that the Drosophila nucleolus contains CTCF, which is bound to transposable element sequences within the rDNA. Reduction in CTCF gene activity results in nucleolar fragmentation and reduced rDNA silencing, as does disruption of poly-ADP-ribosylation thought to be necessary for CTCF nucleolar localization. Our data establish a role for CTCF as a component necessary for proper control of transposable element-laden rDNA transcription and nucleolar stability.
DOI	10.1371/journal.pone.0016401
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Language of Publication	English
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Publication Type	Journal
Abbreviation	PLoS ONE
Title	PLoS ONE
Publication Year	2006-
ISBN/ISSN	1932-6203
Data from Reference
Aberrations (1)
	Df(3L)0463
Alleles (1)
	CTCF^P35.2
Constructs (1)
	P{SUPor-P}
Genes (4)
	CTCF Fib Parg Parp
Insertions (3)
	P{SUPor-P}B486 P{SUPor-P}D285 P{SUPor-P}ROMA