Reference Report

Reference
Citation	Akbar, M.A., Tracy, C., Kahr, W.H., Krämer, H. (2011). The full-of-bacteria gene is required for phagosome maturation during immune defense in Drosophila. J. Cell Biol. 192(3): 383--390. (Export to RIS)
FlyBase ID	FBrf0212933
Publication Type	Research paper
PubMed ID	21282466
PubMed Abstract	Arthrogryposis, renal dysfunction, and cholestasis (ARC) syndrome is a fatal recessive disorder caused by mutations in the VPS33B or VPS16B genes. Both encode homologues of the Vps33p and Vps16p subunits of the HOPS complex necessary for fusions of vacuoles in yeast. Here, we describe a mutation in the full-of-bacteria (fob) gene, which encodes Drosophila Vps16B. Flies null for fob are homozygous viable and fertile. They exhibit, however, a defect in their immune defense that renders them hypersensitive to infections with nonpathogenic bacteria. fob hemocytes (fly macrophages) engulf bacteria but fail to digest them. Phagosomes undergo early steps of maturation and transition to a Rab7-positive stage, but do not mature to fully acidified phagolysosomes. This reflects a specific requirement of fob in the fusion of phagosomes with late endosomes/lysosomes. In contrast, cargo of autophagosomes as well as endosomes exhibit normal lysosomal delivery in fob cells. These findings suggest that defects in phagosome maturation may contribute to symptoms of ARC patients including recurring infections.
DOI	10.1083/jcb.201008119
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	J. Cell Biol.
Title	Journal of Cell Biology
Publication Year	1966-
ISBN/ISSN	0021-9525
Data from Reference
Aberrations (3)
	Df(3R)BSC547 Df(3R)D605 Df(3R)Tl-I
Alleles (8)
	Scer\GAL4^da.G32 Scer\GAL4^srp.Hemo Vps16A^{dsRNA.Scer\UAS.WIZ} Vps16B¹ Vps16B^+t Vps16B^{dsRNA.Scer\UAS.WIZ} Vps16B^{Scer\UAS.T:Hsap\MYC} Vps33B^{dsRNA.Scer\UAS.WIZ}
Constructs (4)
	P{srp.Hemo-GAL4} P{UAS-myc-fob} P{UAS-Vps16A.WIZ} P{UAS-Vps33B.WIZ}
Genes (16)
	AttA CecA1 Def Dpt Dro eater hk Rab5 Rab7 Rbsn-5 Scer\GAL4 Syx7 T:Hsap\MYC Vps16A Vps16B Vps33B
Natural transposons (1)
	P-element