Reference Report

Reference
Citation	Bunt, S., Denholm, B., Skaer, H. (2011). Characterisation of the Drosophila procollagen lysyl hydroxylase, dPlod. Gene Expr. Patterns 11(1-2): 72--78. (Export to RIS)
FlyBase ID	FBrf0213032
Publication Type	Research paper
PubMed ID	20888931
PubMed Abstract	The lysyl hydroxylase (LH) family of enzymes has important roles in the biosynthesis of collagen. In this paper we present the first description of Drosophila LH3 (dPlod), the only lysyl hydroxylase encoded in the fly genome. We have characterised in detail the developmental expression patterns of dPlod RNA and protein during embryogenesis. Consistent with its predicted function as a collagen-modifying enzyme, we find that dPlod is highly expressed in type-IV collagen-producing cells, particularly the haemocytes and fat body. Examination of dPlod subcellular localisation reveals that it is an endoplasmic reticulum resident protein, that partially overlaps with intracellular type-IV collagen. Furthermore, we show that dPlod is required for type-IV collagen secretion from haemocytes and fat body, and thus establish that LH3 enzyme function is conserved across widely separated animal phyla. Our findings, and the new tools we describe, establish the fly as an attractive model in which to study this important collagen biosynthesis enzyme.
DOI	10.1016/j.gep.2010.09.006
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Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Gene Expr. Patterns
Title	Gene expression patterns : GEP
Publication Year	2002-
ISBN/ISSN	1567-133X 1872-7298
Data from Reference
Aberrations (1)
	Df(3L)vin66
Alleles (6)
	Avic\GFP^Scer\UAS.cUa Avic\GFP^vkg-G454 Ecol\lacZ^Scer\UAS.cUa Scer\GAL4^crq.PO Scer\GAL4^Plod-NP5633 Scer\GAL4^srp.Hemo
Constructs (4)
	P{crq-GAL4} P{srp.Hemo-GAL4} P{UAS-GFP.U} P{UAS-lacZ.U}
Genes (6)
	Avic\GFP Cg25C Ecol\lacZ Plod Scer\GAL4 vkg
Insertions (2)
	P{GawB}Plod^NP5633 P{PTT-un1}vkg^G454