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Citation
Tang, H.W., Wang, Y.B., Wang, S.L., Wu, M.H., Lin, S.Y., Chen, G.C. (2011). Atg1-mediated myosin II activation regulates autophagosome formation during starvation-induced autophagy.  EMBO J. 30(4): 636--651.
FlyBase ID
FBrf0213047
Publication Type
Research paper
Abstract

Autophagy is a membrane-mediated degradation process of macromolecule recycling. Although the formation of double-membrane degradation vesicles (autophagosomes) is known to have a central role in autophagy, the mechanism underlying this process remains elusive. The serine/threonine kinase Atg1 has a key role in the induction of autophagy. In this study, we show that overexpression of Drosophila Atg1 promotes the phosphorylation-dependent activation of the actin-associated motor protein myosin II. A novel myosin light chain kinase (MLCK)-like protein, Spaghetti-squash activator (Sqa), was identified as a link between Atg1 and actomyosin activation. Sqa interacts with Atg1 through its kinase domain and is a substrate of Atg1. Significantly, myosin II inhibition or depletion of Sqa compromised the formation of autophagosomes under starvation conditions. In mammalian cells, we found that the Sqa mammalian homologue zipper-interacting protein kinase (ZIPK) and myosin II had a critical role in the regulation of starvation-induced autophagy and mammalian Atg9 (mAtg9) trafficking when cells were deprived of nutrients. Our findings provide evidence of a link between Atg1 and the control of Atg9-mediated autophagosome formation through the myosin II motor protein.

PubMed ID
PubMed Central ID
PMC3041946 (PMC) (EuropePMC)
Related Publication(s)
Note

Unraveling the role of myosin in forming autophagosomes.
Tang and Chen, 2011, Autophagy 7(7): 778--779 [FBrf0216271]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    EMBO J.
    Title
    The EMBO Journal
    Publication Year
    1982-
    ISBN/ISSN
    0261-4189
    Data From Reference