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Hudry, B., Viala, S., Graba, Y., Merabet, S. (2011). Visualization of protein interactions in living Drosophila embryos by the bimolecular fluorescence complementation assay.  BMC Biol. 9(): 5.
FlyBase ID
FBrf0213066
Publication Type
Research paper
Abstract

Protein interactions control the regulatory networks underlying developmental processes. The understanding of developmental complexity will, therefore, require the characterization of protein interactions within their proper environment. The bimolecular fluorescence complementation (BiFC) technology offers this possibility as it enables the direct visualization of protein interactions in living cells. However, its potential has rarely been applied in embryos of animal model organisms and was only performed under transient protein expression levels.Using a Hox protein partnership as a test case, we investigated the suitability of BiFC for the study of protein interactions in the living Drosophila embryo. Importantly, all BiFC parameters were established with constructs that were stably expressed under the control of endogenous promoters. Under these physiological conditions, we showed that BiFC is specific and sensitive enough to analyse dynamic protein interactions. We next used BiFC in a candidate interaction screen, which led to the identification of several Hox protein partners.Our results establish the general suitability of BiFC for revealing and studying protein interactions in their physiological context during the rapid course of Drosophila embryonic development.

PubMed ID
PubMed Central ID
PMC3041725 (PMC) (EuropePMC)
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    BMC Biol.
    Title
    BMC Biology
    ISBN/ISSN
    1741-7007
    Data From Reference
    Alleles (33)
    Genes (11)
    Physical Interactions (9)
    Natural transposons (2)
    Insertions (4)
    Experimental Tools (9)
    Transgenic Constructs (28)