Reference Report

Reference
Citation	Zhou, X., Fabian, L., Bayraktar, J.L., Ding, H.M., Brill, J.A., Chang, H.C. (2011). Auxilin is required for formation of Golgi-derived clathrin-coated vesicles during Drosophila spermatogenesis. Development 138(6): 1111--1120. (Export to RIS)
FlyBase ID	FBrf0213068
Publication Type	Research paper
PubMed ID	21343365
PubMed Abstract	Clathrin has previously been implicated in Drosophila male fertility and spermatid individualization. To understand further the role of membrane transport in this process, we analyzed the phenotypes of mutations in Drosophila auxilin (aux), a regulator of clathrin function, in spermatogenesis. Like partial loss-of-function Clathrin heavy chain (Chc) mutants, aux mutant males are sterile and produce no mature sperm. The reproductive defects of aux males were rescued by male germ cell-specific expression of aux, indicating that auxilin function is required autonomously in the germ cells. Furthermore, this rescue depends on both the clathrin-binding and J domains, suggesting that the ability of Aux to bind clathrin and the Hsc70 ATPase is essential for sperm formation. aux mutant spermatids show a deficit in formation of the plasma membrane during elongation, which probably disrupts the subsequent coordinated migration of investment cones during individualization. In wild-type germ cells, GFP-tagged clathrin localized to clusters of vesicular structures near the Golgi. These structures also contained the Golgi-associated clathrin adaptor AP-1, suggesting that they were Golgi-derived. By contrast, in aux mutant cells, clathrin localized to abnormal patches surrounding the Golgi and its colocalization with AP-1 was disrupted. Based on these results, we propose that Golgi-derived clathrin-positive vesicles are normally required for sustaining the plasma membrane increase necessary for spermatid differentiation. Our data suggest that Aux participates in forming these Golgi-derived clathrin-positive vesicles and that Aux, therefore, has a role in the secretory pathway.
DOI	10.1242/dev.057422
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Alleles (17)
	aux^{CJ.βTub85D.T:Disc\RFP-mRFP} aux^{FL.βTub85D.T:Disc\RFP-mRFP} aux^G257E aux^I670K aux^L78H aux^{ΔC.βTub85D.T:Disc\RFP-mRFP} aux^{ΔJ.βTub85D.T:Disc\RFP-mRFP} aux^{ΔK.βTub85D.T:Disc\RFP-mRFP} Avic\GFP^{βTub85D.T:Myr-Src64B} bam^Δ86 Chc⁴ Clc^{βTub85D.T:Avic\GFP} dj^{T:Avic\GFP-S65T} fws^T:Avic\GFP Rab11^{βTub85D.T:Avic\GFP} Rren\LUC^{βTub85D.T:Avic\GFP} T:Disc\RFP^mRFP
Constructs (11)
	P{dj-GFP.S} P{fws::GFP} P{βTub85D-aux.mRFP.CJ} P{βTub85D-aux.mRFP.FL} P{βTub85D-aux.mRFP.ΔC} P{βTub85D-aux.mRFP.ΔJ} P{βTub85D-aux.mRFP.ΔK} P{βTub85D-Clc.GFP} P{βTub85D-GFP.myr} P{βTub85D-luciferase.GFP} P{βTub85D-Rab11.GFP}
Genes (14)
	aux Avic\GFP bam Chc Clc dj Fas3 fws jar lva Rab11 Rren\LUC T:Avic\GFP vas
Natural transposons (1)
	P-element