Reference Report

Reference
Citation	Guan, Z., Buhl, L.K., Quinn, W.G., Littleton, J.T. (2011). Altered gene regulation and synaptic morphology in Drosophila learning and memory mutants. Learn. Mem. 18(4): 191--206. (Export to RIS)
FlyBase ID	FBrf0213277
Publication Type	Research paper
PubMed ID	21422168
PubMed Abstract	Genetic studies in Drosophila have revealed two separable long-term memory pathways defined as anesthesia-resistant memory (ARM) and long-lasting long-term memory (LLTM). ARM is disrupted in radish (rsh) mutants, whereas LLTM requires CREB-dependent protein synthesis. Although the downstream effectors of ARM and LLTM are distinct, pathways leading to these forms of memory may share the cAMP cascade critical for associative learning. Dunce, which encodes a cAMP-specific phosphodiesterase, and rutabaga, which encodes an adenylyl cyclase, both disrupt short-term memory. Amnesiac encodes a pituitary adenylyl cyclase-activating peptide homolog and is required for middle-term memory. Here, we demonstrate that the Radish protein localizes to the cytoplasm and nucleus and is a PKA phosphorylation target in vitro. To characterize how these plasticity pathways may manifest at the synaptic level, we assayed synaptic connectivity and performed an expression analysis to detect altered transcriptional networks in rutabaga, dunce, amnesiac, and radish mutants. All four mutants disrupt specific aspects of synaptic connectivity at larval neuromuscular junctions (NMJs). Genome-wide DNA microarray analysis revealed ∼375 transcripts that are altered in these mutants, suggesting defects in multiple neuronal signaling pathways. In particular, the transcriptional target Lapsyn, which encodes a leucine-rich repeat cell adhesion protein, localizes to synapses and regulates synaptic growth. This analysis provides insights into the Radish-dependent ARM pathway and novel transcriptional targets that may contribute to memory processing in Drosophila.
DOI	10.1101/lm.2027111
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Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Learn. Mem.
Title	Learning and Memory
Publication Year	1994-
ISBN/ISSN	1072-0502
Data from Reference
Aberrations (1)
	Df(2R)Exel6072
Alleles (19)
	amn¹ dnc¹ Lapsyn^PE Lapsyn^Scer\UAS.cGa Lapsyn^{Scer\UAS.T:Avic\GFP} Lapsyn^zg1 rad¹ rad^Scer\UAS. rut¹ Scer\GAL4^Act.PU Scer\GAL4^elav-C155 Scer\GAL4^how-24B Scer\GAL4^Mef2.PR Scer\GAL4^Mhc.PU Scer\GAL4^αTub84B.PL sei¹ sesB^jive slo^ts1 zyd¹
Constructs (7)
	P{Act-GAL4.U} P{GAL4-Mef2.R} P{Mhc-GAL4.U} P{tubP-GAL4} P{UAS-Lapsyn.GFP} P{UAS-Lapsyn} P{UAS-rad.GFP}
Genes (80)
	5-HT7 aay Ac13E Act88F Adf1 AGO2 amn beat-Vb bnl brp CASK CG3775 CG3822 CG4587 CG6480 CG6852 CG7607 CG11399 CG17746 CG18249 cib cln3 cpb cpx Dab dia dl dnc e Elp3 Fas2 Frq2 GABA-B-R3 glec Grip163 H Idgf1 inaC lap Lapsyn Lk6 MED11 mthl8 Nmda1 nrv2 Nrx-IV Ork1 Pka-C1 Pp2A-29B Ptp61F Pu RabX4 rad RasGAP1 rg Rgl Rim Rpd3 ru rut Scer\GAL4 sei sesB Sh SH3PX1 shakB slo Snap sns spen spir Syt1 Syt7 t T:Avic\GFP Ten-a Tequila tomosyn vig zyd
Insertions (3)
	P{GawB}elav^C155 P{GawB}how^24B P{SUPor-P}Lapsyn^KG09421
Natural transposons (1)
	P-element