|Citation||Daines, Bryce, Emmert, David, Gelbart, William, Chen, Rui (2011.3.30). Addendum concerning analysis of RNA-Seq junctions (Export to RIS)|
|Publication Type||Personal communication to FlyBase|
|Text of Personal Communication||
A small number of junctions predicting very small and very long introns may be mispredictions and should be interpreted with care; these are currently being re-examined. In addition, for ~1900 predicted junctions, the supporting evidence came from a methodology (Wang L, Xi Y, Yu J, Dong L, Yen L, et al. 2010 A Statistical Method for the Detection of Alternative Splicing Using RNA-Seq. PLoS ONE 5(1): e8529. doi:10.1371/journal.pone.0008529) that did not report number of supporting junction-spanning reads. In reality, each of these ~1900 predicted junctions was supported by at least two reads, and so we have noted these by having a total junction-spanning read count of ">1". However, we do not know which developmental stages these supporting reads were in, and so all developmental stages are reported as "0".
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|Language of Publication||English|
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