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Citation
Finan, D., Hartman, M.A., Spudich, J.A. (2011). From the Cover: Proteomics approach to study the functions of Drosophila myosin VI through identification of multiple cargo-binding proteins.  Proc. Natl. Acad. Sci. U.S.A. 108(14): 5566--5571.
FlyBase ID
FBrf0213355
Publication Type
Research paper
Abstract

Myosin VI is a molecular motor implicated in many processes, and it likely associates with a variety of cargoes that specify its functions. Although it is critical to Drosophila development, little is known about its cellular roles. To reveal its involvement in specific pathways, we sought to identify the binding partners of Drosophila myosin VI. We used affinity chromatography and mass spectrometry to discover interacting proteins, which we tested for direct binding. Using this approach, we found that the microtubule-associated protein Cornetto bound myosin VI, and we demonstrated a role for both in secretion of the lipidated morphogen Hedgehog. We also identified a number of other binding proteins, and further characterization of their interactions with myosin VI will advance our understanding of the roles of these complexes in cellular and developmental processes. Thus, our method has provided us the means to gain valuable insight into the multifaceted roles of a motor protein in vivo.

PubMed ID
PubMed Central ID
PMC3078346 (PMC) (EuropePMC)
Related Publication(s)
Note

Multifunctional myosin VI has a multitude of cargoes.
Buss and Kendrick-Jones, 2011, Proc. Natl. Acad. Sci. U.S.A. 108(15): 5927--5928 [FBrf0214226]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Proc. Natl. Acad. Sci. U.S.A.
    Title
    Proceedings of the National Academy of Sciences of the United States of America
    Publication Year
    1915-
    ISBN/ISSN
    0027-8424
    Data From Reference
    Alleles (3)
    Genes (43)
    Physical Interactions (37)
    Cell Lines (1)
    Natural transposons (1)
    Insertions (1)
    Experimental Tools (1)
    Transgenic Constructs (2)