Reference Report

Reference
Citation	Poulton, J.S., Huang, Y.C., Smith, L., Sun, J., Leake, N., Schleede, J., Stevens, L.M., Deng, W.M. (2011). The microRNA pathway regulates the temporal pattern of Notch signaling in Drosophila follicle cells. Development 138(9): 1737--1745. (Export to RIS)
FlyBase ID	FBrf0213494
Publication Type	Research paper
PubMed ID	21447549
PubMed Abstract	Multicellular development requires the correct spatial and temporal regulation of cell division and differentiation. These processes are frequently coordinated by the activities of various signaling pathways such as Notch signaling. From a screen for modifiers of Notch signaling in Drosophila we have identified the RNA helicase Belle, a recently described component of the RNA interference pathway, as an important regulator of the timing of Notch activity in follicle cells. We found that loss of Belle delays activation of Notch signaling, which results in delayed follicle cell differentiation and defects in the cell cycle. Because mutations in well-characterized microRNA components phenocopied the Notch defects observed in belle mutants, Belle might be functioning in the microRNA pathway in follicle cells. The effect of loss of microRNAs on Notch signaling occurs upstream of Notch cleavage, as expression of the constitutively active intracellular domain of Notch in microRNA-defective cells restored proper activation of Notch. Furthermore, we present evidence that the Notch ligand Delta is an important target of microRNA regulation in follicle cells and regulates the timing of Notch activation through cis inhibition of Notch. Here we have uncovered a complex regulatory process in which the microRNA pathway promotes Notch activation by repressing Delta-mediated inhibition of Notch in follicle cells.
DOI	10.1242/dev.059352
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Secondary IDs
Language of Publication	English
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Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Alleles (24)
	Avic\GFP^{Dl.3'UTR.αTub84B} Avic\GFP^Scer\UAS.cUa bel² bel⁵ bel⁶ bel⁷⁴⁴⁰⁷ bel^GD1324 bel^L4740 bel^S047110 bel^unspecified Dcr-1^Q1147X Dcr-2^L811fsX Dl^M1 Dl^RevF10 Dl^Scer\UAS.cHa N^ICN.Scer\UAS pasha^KO piwi¹ Rnor\CD2^HLHmβ.PdC Scer\FLP1^hs.PG Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C} Scer\GAL80^αTub84B.PL Ser^RX106 stau^{αTub67C.T:Avic\GFP-m6}
Constructs (11)
	P{GAL4-Act5C(FRT.CD2).P} P{GD1324} P{HLHmβ-CD2.dC} P{hsFLP} P{lacW} P{tubP-GAL80} P{UAS-Dl.H} P{UAS-GFP.U} P{UAS-N.ICN} P{αTub67C-GFP-stau} P{αTub84B-GFP.Dl.3'UTR}
Genes (19)
	Avic\GFP bel ct CycA CycB Dcr-1 Dcr-2 Dl His3 N pasha peb piwi Rnor\CD2 Scer\FLP1 Scer\GAL4 Scer\GAL80 Ser stau
Insertions (4)
	P{lacW}bel⁷⁴⁴⁰⁷ P{lacW}bel^L4740 P{lacW}bel^S047110 P{PZ}piwi¹
Natural transposons (1)
	P-element