Reference Report

Reference
Citation	Bader, M., Benjamin, S., Wapinski, O.L., Smith, D.M., Goldberg, A.L., Steller, H. (2011). A conserved f box regulatory complex controls proteasome activity in Drosophila. Cell 145(3): 371--382. (Export to RIS)
FlyBase ID	FBrf0213568
Publication Type	Research paper
PubMed ID	21529711
PubMed Abstract	The ubiquitin-proteasome system catalyzes the degradation of intracellular proteins. Although ubiquitination of proteins determines their stabilities, there is growing evidence that proteasome function is also regulated. We report the functional characterization of a conserved proteasomal regulatory complex. We identified DmPI31 as a binding partner of the F box protein Nutcracker, a component of an SCF ubiquitin ligase (E3) required for caspase activation during sperm differentiation in Drosophila. DmPI31 binds Nutcracker via a conserved mechanism that is also used by mammalian FBXO7 and PI31. Nutcracker promotes DmPI31 stability, which is necessary for caspase activation, proteasome function, and sperm differentiation. DmPI31 can activate 26S proteasomes in vitro, and increasing DmPI31 levels suppresses defects caused by diminished proteasome activity in vivo. Furthermore, loss of DmPI31 function causes lethality, cell-cycle abnormalities, and defects in protein degradation, demonstrating that DmPI31 is physiologically required for normal proteasome activity.
DOI	10.1016/j.cell.2011.03.021
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Cell
Title	Cell
Publication Year	1974-
ISBN/ISSN	0092-8674
Data from Reference
Aberrations (2)
	Df(2R)BSC199 Df(2R)ED2247
Alleles (20)
	ntc^dj.T:Saur\A ntc^f07259 ntc^ms771 ntc^V-E.hsp83 ntc^WT.hsp83 ntc^{ΔF.dj.T:Saur\A} osk⁸ osk^CE4 PI31^GD13764 PI31^KO PI31^{Scer\UAS.P\T.T:Ivir\HA1} PI31^{Scer\UAS.T:Ivir\HA1} PI31^{T:Disc\RFP-mCherry} Prosβ2^1.Scer\UAS Prosβ6^1.B.Scer\UAS Scer\GAL4^Act.PU Scer\GAL4^GMR.PU Scer\GAL4^tub.PU Scer\GAL80^ts.αTub84B T:Disc\RFP^mCherry
Constructs (14)
	P{Act-GAL4.U} P{dj-PrA-ntc.ΔF} P{dj-PrA-ntc} P{GD13764} P{GMR-GAL4.U} P{Hsp83-ntc.V-E} P{Hsp83-ntc.WT} P{PI31.mCherry} P{tub-GAL4.U} P{tubP-GAL80^ts} P{UAS-PI31.HA} P{UASp-PI31.HA} P{UAS-Pros26¹.B} P{UAS-Prosβ2¹}
Genes (12)
	CycB ntc osk PI31 Prosα1 Prosα7 Prosβ2 Prosβ6 Scer\GAL4 Scer\GAL80 T:Ivir\HA1 vas
Insertions (1)
	PBac{WH}ntc^f07259
Natural transposons (1)
	P-element