Reference Report

Reference
Citation	Sun, Y., Yan, Y., Denef, N., Schüpbach, T. (2011). Regulation of somatic myosin activity by Protein Phosphatase 1{beta} controls Drosophila oocyte polarization. Development 138(10): 1991--2001. (Export to RIS)
FlyBase ID	FBrf0213586
Publication Type	Research paper
PubMed ID	21490061
PubMed Abstract	The Drosophila body axes are established in the oocyte during oogenesis. Oocyte polarization is initiated by Gurken, which signals from the germline through the epidermal growth factor receptor (Egfr) to the posterior follicle cells (PFCs). In response the PFCs generate an unidentified polarizing signal that regulates oocyte polarity. We have identified a loss-of-function mutation of flapwing, which encodes the catalytic subunit of protein phosphatase 1β (PP1β) that disrupts oocyte polarization. We show that PP1β, by regulating myosin activity, controls the generation of the polarizing signal. Excessive myosin activity in the PFCs causes oocyte mispolarization and defective Notch signaling and endocytosis in the PFCs. The integrated activation of JAK/STAT and Egfr signaling results in the sensitivity of PFCs to defective Notch. Interestingly, our results also demonstrate a role of PP1β in generating the polarizing signal independently of Notch, indicating a direct involvement of somatic myosin activity in axis formation.
DOI	10.1242/dev.062190
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Alleles (30)
	btl::Egfr^{Scer\UAS.T:λ\cI-DD} Ecol\lacZ^CycE.16.4 Ecol\lacZ^ex-697 Ecol\lacZ^kek1-BB142 Ecol\lacZ^mirr-6D1 Ecol\lacZ^{P\T.T:Khc-606} Ecol\lacZ^pnt-S099812 Ecol\lacZ^th-j5C8 flw^FP41 flw^{Scer\UAS.T:Ivir\HA1} Mbs^T541 Mbs^T666 Mbs^T791 MYPT-75D^{F117A.Scer\UAS} MYPT-75D^{Scer\UAS.P\T.cKa} N^{ECN.Scer\UAS.cGa} N^ICN.Scer\UAS N^Scer\UAS.cSb os^Scer\UAS.cZa Rab5^{Scer\UAS.T:Avic\GFP-EGFP} Rab7^{Scer\UAS.T:Avic\GFP} Rab11^{Scer\UAS.T:Avic\GFP} Rho1^E3.10 RhoGEF2⁰⁴²⁹¹ rok^CAT.Scer\UAS rok^{CAT-KG.Scer\UAS} Scer\GAL4^Act5C.PU Scer\GAL4^e22c Scer\GAL4^{Scer\FRT.Act5C} zip¹
Constructs (17)
	P{Act5C-GAL4.U} P{AyGAL4} P{CycE-lacZ.16.4} P{KZ.TRAP} P{UAS-Egfr.λtop} P{UAS-flw.HA} P{UAS-GFP-Rab5} P{UAS-MYPT-75D.F117A} P{UAS-N.ECN.G} P{UAS-N.ICN} P{UAS-N⁺.Sb} P{UAS-os.Z} P{UASp-MYPT-75D.K} P{UAS-Rab7.GFP} P{UAS-Rab11.GFP} P{UAS-rok.CAT} P{UAS-rok.CAT-KG}
Genes (38)
	aPKC arm baz btl::Egfr ct CycE dlg1 dome Ecol\lacZ Egfr ex eya flw grk His3 kek1 lva Mbs mirr MYPT-75D N os osk Patj peb pnt Rab5 Rab7 Rab11 Rho1 RhoGEF2 rok Scer\GAL4 shg sqh stau th zip
Insertions (7)
	P{en2.4-GAL4}e22c P{lacW}BB142 P{lacW}ex⁶⁹⁷ P{lacW}mirr^6D1 P{lacW}pnt^S099812 P{lacW}th^j5C8 P{PZ}RhoGEF2⁰⁴²⁹¹