Reference Report

Reference
Citation	Neumüller, R.A., Richter, C., Fischer, A., Novatchkova, M., Neumüller, K.G., Knoblich, J.A. (2011). Genome-Wide Analysis of Self-Renewal in Drosophila Neural Stem Cells by Transgenic RNAi. Cell Stem Cell 8(5): 580--593. (Export to RIS)
FlyBase ID	FBrf0213621
Publication Type	Research paper
PubMed ID	21549331
PubMed Abstract	The balance between stem cell self-renewal and differentiation is precisely controlled to ensure tissue homeostasis and prevent tumorigenesis. Here we use genome-wide transgenic RNAi to identify 620 genes potentially involved in controlling this balance in Drosophila neuroblasts. We quantify all phenotypes and derive measurements for proliferation, lineage, cell size, and cell shape. We identify a set of transcriptional regulators essential for self-renewal and use hierarchical clustering and integration with interaction data to create functional networks for the control of neuroblast self-renewal and differentiation. Our data identify key roles for the chromatin remodeling Brm complex, the spliceosome, and the TRiC/CCT-complex and show that the alternatively spliced transcription factor Lola and the transcriptional elongation factors Ssrp and Barc control self-renewal in neuroblast lineages. As our data are strongly enriched for genes highly expressed in murine neural stem cells, they are likely to provide valuable insights into mammalian stem cell biology as well.
DOI	10.1016/j.stem.2011.02.022
Related Publication(s)
Supplementary material	Supplemental Table S1. [FBrf0214431]
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Secondary IDs
Language of Publication	English
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Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Cell Stem Cell
Title	Cell Stem Cell
Publication Year	2007--
ISBN/ISSN	1934-5909
Data from Reference
Alleles (19)
	barc^Scer\UAS.cNa barc^{Scer\UAS.RNAi-res} brat^GD7073 brat^KK113206 dia^GD9442 dia^KK101745 lola^GD4071 lola^GD6806 lola^GD9568 lola^KK110256 Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Nop56^GD8135 Nop56^KK102280 Orc2^GD4122 Orc2^KK102852 Scer\GAL4^ase.neuro Scer\GAL4^insc-Mz1407 Scer\GAL4^wor.PA Scer\GAL80^ase.PN
Constructs (18)
	P{ase-GAL4} P{ase-GAL80.N} P{GD4071} P{GD4122} P{GD6806} P{GD7073} P{GD8135} P{GD9442} P{GD9568} P{KK101745} P{KK102280} P{KK102852} P{KK110256} P{KK113206} P{UAS-barc.N} P{UAS-barc.RNAi-res} P{UAS-mCD8::GFP.L} P{wor.GAL4.A}
Genes (244)
	All Genes relating to this reference
Insertions (1)
	P{GawB}insc^Mz1407
Natural transposons (1)
	P-element