Reference Report

Reference
Citation	Chan, Y.S., Naujoks, D.A., Huen, D.S., Russell, S. (2011). Insect Population Control by Homing Endonuclease-Based Gene Drive: An Evaluation in Drosophila melanogaster. Genetics 188(1): 33--44. (Export to RIS)
FlyBase ID	FBrf0213627
Publication Type	Research paper
PubMed ID	21368273
PubMed Abstract	Insects play a major role as vectors of human disease as well as causing significant agricultural losses. Harnessing the activity of customized homing endonuclease genes (HEGs) has been proposed as a method for spreading deleterious mutations through populations with a view to controlling disease vectors. Here, we demonstrate the feasibility of this method in Drosophila melanogaster, utilizing the well-characterized HEG, I-SceI. In particular, we show that high rates of homing can be achieved within spermatogonia and in the female germline. We show that homed constructs continue to exhibit HEG activity in the subsequent generation and that the ectopic homing events required for initiating the strategy occur at an acceptable rate. We conclude that the requirements for successful deployment of a HEG-based gene drive strategy can be satisfied in a model dipteran and that there is a reasonable prospect of the method working in other dipterans. In characterizing the system we measured repair outcomes at the spermatogonial, spermatocyte, and spermatid stages of spermatogenesis. We show that homologous recombination is restricted to spermatogonia and that it immediately ceases when they become primary spermatocytes, indicating that the choice of DNA repair pathway in the Drosophila testis can switch abruptly during differentiation.
DOI	10.1534/genetics.111.127506
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Secondary IDs
Language of Publication	English
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Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Alleles (15)
	Avic\GFP^{DarkLime.3xP3.Scer\SceI.RS} Avic\GFP^E.3xP3 Avic\GFP^{Lemon.3xP3.Scer\SceI.RS} Disc\RFP^cCa Scer\SCEI^{Act5C.P\T.HEG-1} Scer\SCEI^{Act5C.P\T.HEG-2} Scer\SCEI^aly.HEG-1 Scer\SCEI^bam.HEG-2 Scer\SCEI^Hsp26.HEG-1 Scer\SCEI^Hsp70.HEG-1 Scer\SCEI^Mst87F.HEG-1 Scer\SCEI^vas.HEG-2 Scer\SCEI^{βTub85D.HEG-1} w^+* w^wDarkLime
Constructs (12)
	P{Act5C-P-SCEI.HEG-1} P{Act5C-P-SCEI.HEG-2} P{aly-SCEI.HEG-1} P{bam-SCEI.HEG-2} P{DarkLime} P{Hsp26-SCEI.HEG-1} P{Hsp70-SCEI.HEG-1} P{Lemon} P{Mst87F-SCEI.HEG-1} P{vas-SCEI.HEG-2} P{wDarkLime} P{βTub85D-SCEI.HEG-1}
Genes (11)
	Act5C aly Avic\GFP bam Disc\RFP Hsp26 Mst87F Scer\SCEI vas w βTub85D
Insertions (10)
	P{Act5C-P-SCEI.HEG-2}attP2 P{aly-SCEI.HEG-1}attP2 P{CaryP}attP2 P{DarkLime}attP2 P{Hsp26-SCEI.HEG-1}attP2 P{Hsp70-SCEI.HEG-1}attP2 P{Mst87F-SCEI.HEG-1}attP2 P{vas-SCEI.HEG-2}attP2 P{wDarkLime}attP2 P{βTub85D-SCEI.HEG-1}attP2
Natural transposons (1)
	P-element