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White, A.E., Burch, B.D., Yang, X.C., Gasdaska, P.Y., Dominski, Z., Marzluff, W.F., Duronio, R.J. (2011). Drosophila histone locus bodies form by hierarchical recruitment of components.  J. Cell Biol. 193(4): 677--694.
FlyBase ID
FBrf0213662
Publication Type
Research paper
Abstract

Nuclear bodies are protein- and RNA-containing structures that participate in a wide range of processes critical to genome function. Molecular self-organization is thought to drive nuclear body formation, but whether this occurs stochastically or via an ordered, hierarchical process is not fully understood. We addressed this question using RNAi and proteomic approaches in Drosophila melanogaster to identify and characterize novel components of the histone locus body (HLB), a nuclear body involved in the expression of replication-dependent histone genes. We identified the transcription elongation factor suppressor of Ty 6 (Spt6) and a homologue of mammalian nuclear protein of the ataxia telangiectasia-mutated locus that is encoded by the homeotic gene multisex combs (mxc) as novel HLB components. By combining genetic manipulation in both cell culture and embryos with cytological observations of Mxc, Spt6, and the known HLB components, FLICE-associated huge protein, Mute, U7 small nuclear ribonucleoprotein, and MPM-2 phosphoepitope, we demonstrated sequential recruitment and hierarchical dependency for localization of factors to HLBs during development, suggesting that ordered assembly can play a role in nuclear body formation.

PubMed ID
PubMed Central ID
PMC3166876 (PMC) (EuropePMC)
Related Publication(s)
Note

Seed and grow: a two-step model for nuclear body biogenesis.
Dundr, 2011, J. Cell Biol. 193(4): 605--606 [FBrf0214473]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    J. Cell Biol.
    Title
    Journal of Cell Biology
    Publication Year
    1966-
    ISBN/ISSN
    0021-9525
    Data From Reference
    Alleles (7)
    Genes (51)
    Physical Interactions (3)
    Cell Lines (1)
    Natural transposons (1)
    Insertions (1)
    Experimental Tools (1)
    Transgenic Constructs (1)