Reference Report

Reference
Citation	Layalle, S., Volovitch, M., Mugat, B., Bonneaud, N., Parmentier, M.L., Prochiantz, A., Joliot, A., Maschat, F. (2011). Engrailed homeoprotein acts as a signaling molecule in the developing fly. Development 138(11): 2315--2323. (Export to RIS)
FlyBase ID	FBrf0213689
Publication Type	Research paper
PubMed ID	21558379
PubMed Abstract	Homeodomain transcription factors classically exert their morphogenetic activities through the cell-autonomous regulation of developmental programs. In vertebrates, several homeoproteins have also been shown to have direct non-cell-autonomous activities in the developing nervous system. We present the first in vivo evidence for homeoprotein signaling in Drosophila. Focusing on wing development as a model, we first demonstrate that the homeoprotein Engrailed (En) is secreted. Using single-chain anti-En antibodies expressed under the control of a variety of promoters, we delineate the wing territories in which secreted En acts. We show that En is a short-range signaling molecule that participates in anterior crossvein development, interacting with the Dpp signaling pathway. This report thus suggests that direct signaling with homeoproteins is an evolutionarily conserved phenomenon that is not restricted to neural tissues and involves interactions with bona fide signal transduction pathways.
DOI	10.1242/dev.057059
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Secondary IDs
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Aberrations (2)
	Df(2R)en-SFX31 Df(2R)Pu-D17
Alleles (15)
	Avic\GFP^Scer\UAS.cUa dpp^d6 Ecol\lacZ^ptc-H84 en^{4F11ScFv.Scer\UAS.T:Hsap\MYC} en⁵⁴ en^{dsRNA.Scer\UAS} en^{Scer\UAS.T:Avic\GFP-EGFP} en^{SP4F11ScFv.Scer\UAS.T:Hsap\MYC} Scer\GAL4^Bx-MS1096 Scer\GAL4^dpp.blk1 Scer\GAL4^en-e16E Scer\GAL4^hh-Gal4 Scer\GAL4^ptc-559.1 T:Avic\GFP^EGFP tkv⁴
Constructs (6)
	P{GAL4-dpp.blk1} P{UAS-4F11ScFv.MYC} P{UAS-en.eGFP} P{UAS-en.RNAi} P{UAS-en.SP4F11ScFv.MYC} P{UAS-GFP.U}
Genes (12)
	Avic\GFP bs cv-2 dpp Ecol\lacZ en inv Mad ptc Scer\GAL4 T:Hsap\MYC tkv
Insertions (5)
	P{A92}ptc^H84 P{en2.4-GAL4}e16E P{GAL4}hh^Gal4 P{GawB}Bx^MS1096 P{GawB}ptc^559.1
Natural transposons (1)
	P-element