Reference Report

Reference
Citation	Zappia, M.P., Brocco, M.A., Billi, S.C., Frasch, A.C., Ceriani, M.F. (2011). M6 membrane protein plays an essential role in Drosophila oogenesis. PLoS ONE 6(5): e19715. (Export to RIS)
FlyBase ID	FBrf0213770
Publication Type	Research paper
PubMed ID	21603606
PubMed Abstract	We had previously shown that the transmembrane glycoprotein M6a, a member of the proteolipid protein (PLP) family, regulates neurite/filopodium outgrowth, hence, M6a might be involved in neuronal remodeling and differentiation. In this work we focused on M6, the only PLP family member present in Drosophila, and ortholog to M6a. Unexpectedly, we found that decreased expression of M6 leads to female sterility. M6 is expressed in the membrane of the follicular epithelium in ovarioles throughout oogenesis. Phenotypes triggered by M6 downregulation in hypomorphic mutants included egg collapse and egg permeability, thus suggesting M6 involvement in eggshell biosynthesis. In addition, RNAi-mediated M6 knockdown targeted specifically to follicle cells induced an arrest of egg chamber development, revealing that M6 is essential in oogenesis. Interestingly, M6-associated phenotypes evidenced abnormal changes of the follicle cell shape and disrupted follicular epithelium in mid- and late-stage egg chambers. Therefore, we propose that M6 plays a role in follicular epithelium maintenance involving membrane cell remodeling during oogenesis in Drosophila.
DOI	10.1371/journal.pone.0019715
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Language of Publication	English
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Publication Type	Journal
Abbreviation	PLoS ONE
Title	PLoS ONE
Publication Year	2006-
ISBN/ISSN	1932-6203
Data from Reference
Alleles (25)
	Avic\GFP^M6-CA06602 M6^BG00390 M6^EY07032 M6^KK109436 M6^MB02608 M6^NIG.7540R M6^Δ01-50 M6^Δ01-470 M6^Δ01-580 M6^Δ01-730 M6^Δ01-3200 M6^Δ01-5100 M6^Δ01-7500 M6^Δ01-rev Scer\GAL4^55B Scer\GAL4^198Y Scer\GAL4^c204 Scer\GAL4^c329b Scer\GAL4^c355 Scer\GAL4^da.G32 Scer\GAL4^dm-PG45 Scer\GAL4^e22c Scer\GAL4^slbo.2.6 Scer\GAL4^T155 Scer\GAL4^αTub84B.PL
Constructs (6)
	P{?EPgy2} P{GAL4-da.G32} P{GAL4-slbo.2.6} P{KK109436} P{NIG.7540R} P{tubP-GAL4}
Genes (10)
	arm Avic\GFP Cad99C Fas3 Glg1 M6 Scer\GAL4 Vm26Aa Vm26Ab yl
Insertions (19)
	Mi{ET1}M6^MB02608 P{?EPgy2}M6^Δ01-50 P{?EPgy2}M6^Δ01-580 P{?EPgy2}M6^Δ01-730 P{?EPgy2}M6^Δ01-3200 P{?EPgy2}M6^Δ01-5100 P{?EPgy2}M6^Δ01-7500 P{?EPgy2}M6^Δ01-470 P{en2.4-GAL4}e22c P{EPgy2}M6^EY07032 P{GawB}198Y P{GawB}c204 P{GawB}c329b P{GawB}c355 P{GawB}dm^PG45 P{GawB}Eip63F-1^55B P{GawB}T155 P{GT1}M6^BG00390 P{PTT-GA}M6^CA06602
Natural transposons (1)
	P-element