Reference Report

Reference
Citation	Wu, C.L., Shih, M.F., Lai, J.S., Yang, H.T., Turner, G.C., Chen, L., Chiang, A.S. (2011). Heterotypic Gap Junctions between Two Neurons in the Drosophila Brain Are Critical for Memory. Curr. Biol. 21(10): 848--854. (Export to RIS)
FlyBase ID	FBrf0213804
Publication Type	Research paper
PubMed ID	21530256
PubMed Abstract	Gap junctions play an important role in the regulation of neuronal metabolism and homeostasis by serving as connections that enable small molecules to pass between cells and synchronize activity between cells. Although recent studies have linked gap junctions to memory formation, it remains unclear how they contribute to this process. Gap junctions are hexameric hemichannels formed from the connexin and pannexin gene families in chordates and the innexin (inx) gene family in invertebrates. Here we show that two modulatory neurons, the anterior paired lateral (APL) neuron and the dorsal paired medial (DPM) neuron, form heterotypic gap junctions within the mushroom body (MB), a learning and memory center in the Drosophila brain. Using RNA interference-mediated knockdowns of inx7 and inx6 in the APL and DPM neurons, respectively, we found that flies showed normal olfactory associative learning and intact anesthesia-resistant memory (ARM) but failed to form anesthesia-sensitive memory (ASM). Our results reveal that the heterotypic gap junctions between the APL and DPM neurons are an essential part of the MB circuitry for memory formation, potentially constituting a recurrent neural network to stabilize ASM.
DOI	10.1016/j.cub.2011.02.041
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Curr. Biol.
Title	Current Biology
Publication Year	1991-
ISBN/ISSN	0960-9822
Data from Reference
Alleles (20)
	Avic\GFP^{GCaMP1.6.Scer\UAS} Disc\RFP^Mef2.mb247 Ecol\lexA^L0111 Inx2^KK111067 Inx3^GD14965 Inx5^GD1177 Inx6^GD3692 Inx6^GD16939 Inx7^KK112684 Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} ogre^KK104770 Rnor\CD2^{Ecol\lexAop.T:Avic\GFP} Scer\GAL4⁵⁰¹⁵ Scer\GAL4^c316 Scer\GAL4^GH146 Scer\GAL80^Cha.PK Scer\GAL80^ts.αTub84B shakB^GD12666 zpg^GD2338 Zzzz\KO^Scer\UAS.cWa
Constructs (17)
	P{Cha-GAL80.K} P{GD1177} P{GD2338} P{GD3692} P{GD12666} P{GD14965} P{GD16939} P{KK104770} P{KK111067} P{KK112684} P{lexAop-rCD2-GFP} P{mb247-DsRed} P{tubP-GAL80^ts} P{UAS-G-CaMP.1.6} P{UAS-lexA-GAD} P{UAS-mCD8::GFP.L} P{UAS-mKO.W}
Genes (15)
	Disc\RFP dlg1 Inx2 Inx3 Inx5 Inx6 Inx7 Mmus\Cd8a ogre Rnor\CD2 Scer\GAL4 Scer\GAL80 shakB zpg Zzzz\KO
Insertions (4)
	P{GawB}5015 P{GawB}c316 P{GawB}GH146 P{UAS-lexA-GAD}L0111
Natural transposons (1)
	P-element
Transcripts (4)
	Ecol\lexA^L0111RA Scer\GAL4⁵⁰¹⁵RA Scer\GAL4^c316RA Scer\GAL4^GH146RA