Reference Report

Reference
Citation	Reyes-Carmona, S., Valadéz-Graham, V., Aguilar-Fuentes, J., Zurita, M., León-Del-Río, A. (2011). Trafficking and chromatin dynamics of holocarboxylase synthetase during development of Drosophila melanogaster. Molec. Genet. Metab. 103(3): 240--248. (Export to RIS)
FlyBase ID	FBrf0213836
Publication Type	Research paper
PubMed ID	21463962
PubMed Abstract	This work examines the cellular localization of holocarboxylase synthetase (HCS) and its association to chromatin during different stages of development of Drosophila melanogaster. While HCS is well known for its role in the attachment of biotin to biotin-dependent carboxylase, it also regulates the transcription of HCS and carboxylases genes by triggering a cGMP-dependent signal transduction cascade. Further, its presence in the nucleus of cells suggests additional regulatory roles, but the mechanism involved has remained elusive. In this study, we show in D. melanogaster that HCS migrates to the nucleus at the gastrulation stage. In polytene chromosomes, it is associated to heterochromatin bands where it co-localizes with histone 3 trimethylated at lysine 9 (H3K9met3) but not with the euchromatin mark histone 3 acetylated at lysine 9 (H3K9ac). Further, we demonstrate the association of HCS with the hsp70 promoter by immunofluorescence and chromatin immuno-precipitation (ChIP) of associated DNA sequences. We demonstrate the occupancy of HCS to the core promoter region of the transcriptionally inactive hsp70 gene. On heat-shock activation of the hsp70 promoter, HCS is displaced and the promoter region becomes enriched with the TFIIH subunits XPD and XPB and elongating RNA pol II, the latter also demonstrated using ChIP assays. We suggest that HCS may have a role in the repression of gene expression through a mechanism involving its trafficking to the nucleus and interaction with heterochromatic sites coincident with H3K9met3.
DOI	10.1016/j.ymgme.2011.03.005
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Molec. Genet. Metab.
Title	Molecular genetics and metabolism
Publication Year	1998-
ISBN/ISSN	1096-7192
Data from Reference
Genes (2)
	Hcs His3