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Burgess, J., Jauregui, M., Tan, J., Rollins, J., Lallet, S., Leventis, P.A., Boulianne, G.L., Chang, H.C., Le Borgne, R., Krämer, H., Brill, J.A. (2011). AP-1 and clathrin are essential for secretory granule biogenesis in Drosophila.  Mol. Biol. Cell 22(12): 2094--2105.
FlyBase ID
FBrf0213890
Publication Type
Research paper
Abstract

Regulated secretion of hormones, digestive enzymes, and other biologically active molecules requires the formation of secretory granules. Clathrin and the clathrin adaptor protein complex 1 (AP-1) are necessary for maturation of exocrine, endocrine, and neuroendocrine secretory granules. However, the initial steps of secretory granule biogenesis are only minimally understood. Powerful genetic approaches available in the fruit fly Drosophila melanogaster were used to investigate the molecular pathway for biogenesis of the mucin-containing "glue granules" that form within epithelial cells of the third-instar larval salivary gland. Clathrin and AP-1 colocalize at the trans-Golgi network (TGN) and clathrin recruitment requires AP-1. Furthermore, clathrin and AP-1 colocalize with secretory cargo at the TGN and on immature granules. Finally, loss of clathrin or AP-1 leads to a profound block in secretory granule formation. These findings establish a novel role for AP-1- and clathrin-dependent trafficking in the biogenesis of mucin-containing secretory granules.

PubMed ID
PubMed Central ID
PMC3113773 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Mol. Biol. Cell
    Title
    Molecular Biology of the Cell
    Publication Year
    1992-
    ISBN/ISSN
    1059-1524
    Data From Reference
    Alleles (14)
    Genes (13)
    Natural transposons (1)
    Insertions (2)
    Experimental Tools (3)
    Transgenic Constructs (8)