Reference Report

Reference
Citation	Kawasaki, F., Iyer, J., Posey, L.L., Sun, C.E., Mammen, S.E., Yan, H., Ordway, R.W. (2011). The DISABLED protein functions in CLATHRIN-mediated synaptic vesicle endocytosis and exoendocytic coupling at the active zone. Proc. Natl. Acad. Sci. U.S.A. 108(25): E222--E229. (Export to RIS)
FlyBase ID	FBrf0213990
Publication Type	Research paper
PubMed ID	21606364
PubMed Abstract	Members of the DISABLED (DAB) family of proteins are known to play a conserved role in endocytic trafficking of cell surface receptors by functioning as monomeric CLATHRIN-associated sorting proteins that recruit cargo proteins into endocytic vesicles. Here, we report a Drosophila disabled mutant revealing a novel role for DAB proteins in chemical synaptic transmission. This mutant exhibits impaired synaptic function, including a rapid activity-dependent reduction in neurotransmitter release and disruption of synaptic vesicle endocytosis. In presynaptic boutons, Drosophila DAB and CLATHRIN were highly colocalized within two distinct classes of puncta, including relatively dim puncta that were located at active zones and may reflect endocytic mechanisms operating at neurotransmitter release sites. Finally, broader analysis of endocytic proteins, including DYNAMIN, supported a general role for CLATHRIN-mediated endocytic mechanisms in rapid clearance of neurotransmitter release sites for subsequent vesicle priming and refilling of the release-ready vesicle pool.
DOI	10.1073/pnas.1102231108
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Proc. Natl. Acad. Sci. U.S.A.
Title	Proceedings of the National Academy of Sciences of the United States of America
Publication Year	1915-
ISBN/ISSN	0027-8424
Data from Reference
Aberrations (1)
	Df(3L)Exel6130
Alleles (13)
	cac^TS2 Chc^GD14095 Dab^EC1 Dab^EY10190 Dab^Scer\UAS.cWa Dab^{Scer\UAS.T:Avic\GFP-EGFP} Dap160^{Scer\UAS.T:Avic\GFP-EGFP} Dap160^Δ1 Dap160^Δ6 Scer\GAL4^Appl.G1a shi¹ Snap25^ts T:Avic\GFP^EGFP
Constructs (5)
	P{Appl-GAL4.G1a} P{GD14095} P{UAS-Dab.W} P{UAS-Dab-EGFP} P{UAS-Dap160-EGFP}
Genes (8)
	cac Chc Clc Dab Dap160 Scer\GAL4 shi Snap25
Insertions (1)
	P{EPgy2}Dab^EY10190
Natural transposons (1)
	P-element