Reference Report

Reference
Citation	Fullaondo, A., García-Sánchez, S., Sanz-Parra, A., Recio, E., Lee, S.Y., Gubb, D. (2011). Spn1 Regulates the GNBP3-Dependent Toll Signaling Pathway in Drosophila melanogaster. Mol. Cell. Biol. 31(14): 2960--2972. (Export to RIS)
FlyBase ID	FBrf0214037
Publication Type	Research paper
PubMed ID	21576362
PubMed Abstract	The Drosophila genome encodes 29 serpins, most of unknown function. We show here that Spn1 is an active protease inhibitor of the serpin superfamily. Spn1 inhibits trypsin in vitro and regulates the Toll-mediated immune response in vivo. Expression of the Toll-dependent transcripts Drosomycin and IM1 is increased in Spn1 null mutants. Overexpression of Spn1 reduces the induction of Drosomycin upon immune challenge with fungi but not Gram-positive bacteria. Similar reductions in Drosomycin levels are observed in the psh, spz, and grass mutants of the Toll signaling pathway. These results support a role of Spn1 as a repressor of Toll activation upon fungal infection. Epistatic analysis places Spn1 upstream of Spätzle processing enzyme and Grass, in the fungal cell wall-activated side branch of the pathway. Overexpression of the pattern recognition receptor GNBP3 activates the β-1,3-glucan-sensitive side branch of the Toll pathway. The resultant increased Drosomycin level is reduced by concomitant overexpression of Spn1, confirming that Spn1 regulates the fungal cell wall side branch. Spn1 null mutants show altered susceptibility to fungal infection compared to the wild type, demonstrating a requirement for Spn1 in the fine regulation of the immune response.
DOI	10.1128/MCB.01397-10
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Mol. Cell. Biol.
Title	Molecular and Cellular Biology
Publication Year	1981-
ISBN/ISSN	0270-7306
Data from Reference
Aberrations (1)
	Df(3R)mbc-R1
Alleles (21)
	Dif¹ Drs^{T:Avic\GFP-S65T} GNBP1^Scer\UAS.cGa GNBP3^hades GNBP3^Scer\UAS.cGa grass^Herrade grass^Scer\UAS.cCa modSP¹ modSP^Scer\UAS.cBa PGRP-SA^Scer\UAS.cMa psh⁴ Rel^E20 Scer\GAL4^ap-md544 Scer\GAL4^c564 Scer\GAL4^yolk SPE^{active.Scer\UAS.T:SV5\V5} SPE^Pasteur Spn42Dd^NIG.cUa Spn42Dd^oka Spn42Dd^Scer\UAS.cFa spz⁴
Constructs (6)
	P{Drs-GFP.JM804} P{NIG.Spn1.U} P{UAS-SPE.a} P{UAS-Spn1.F} P{yolk-GAL4} PBac{WHr}
Genes (15)
	Dif Dpt Drs GNBP1 GNBP3 grass IM1 modSP PGRP-SA psh Rel Scer\GAL4 SPE Spn42Dd spz
Insertions (5)
	P{GawB}ap^md544 P{GawB}c564 PBac{WH}f04856 PBac{WH}Spn42Dd^f02145 PBac{WHr}Spn42Dd^oka
Natural transposons (1)
	P-element