Reference Report

Reference
Citation	Jia, H., Liu, Y., Xia, R., Tong, C., Yue, T., Jiang, J., Jia, J. (2010). Casein kinase 2 promotes Hedgehog signaling by regulating both smoothened and Cubitus interruptus. J. Biol. Chem. 285(48): 37218--37226. (Export to RIS)
FlyBase ID	FBrf0214177
Publication Type	Research paper
PubMed ID	20876583
PubMed Abstract	Casein kinase 2 (CK2) is a typical serine/threonine kinase consisting of α and β subunits and has been implicated in many cellular and developmental processes. In this study, we demonstrate that CK2 is a positive regulator of the Hedgehog (Hh) signal transduction pathway. We found that inactivation of CK2 by CK2β RNAi enhances the loss-of-Hh wing phenotype induced by a dominant negative form of Smoothened (Smo). CK2β RNAi attenuates Hh-induced Smo accumulation and down-regulates Hh target gene expression, whereas increasing CK2 activity by coexpressing CK2α and CK2β increases Smo accumulation and induces ectopic Hh target gene expression. We identified the serine residues in Smo that can be phosphorylated by CK2 in vitro. Mutating these serine residues attenuates the ability of Smo to transduce high level Hh signaling activity in vivo. Furthermore, we found that CK2 plays an additional positive role downstream of Smo by regulating the stability of full-length Cubitus interruptus (Ci). CK2β RNAi promotes Ci degradation whereas coexpressing CK2α and CK2β increases the half-life of Ci. We showed that CK2 prevents Ci ubiquitination and degradation by the proteasome. Thus, CK2 promotes Hh signaling activity by regulating multiple pathway components.
DOI	10.1074/jbc.M110.174565
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Biol. Chem.
Title	Journal of Biological Chemistry
Publication Year	1905-
ISBN/ISSN	0021-9258
Data from Reference
Alleles (31)
	Avic\GFP^Scer\UAS.cUa ci^{-3P.Scer\UAS.W.T:Ivir\HA1} ci^{Scer\UAS.W.T:Ivir\HA1} CkIIα^{KM.Scer\UAS.T:Zzzz\FLAG} CkIIα^{Scer\UAS.T:Zzzz\FLAG} CkIIα^Tik CkIIβ^{dsRNA.cJa.Scer\UAS} CkIIβ^GD8571 CkIIβ^KK109263 CkIIβ^{Scer\UAS.T:Zzzz\FLAG} Dcr-2^Scer\UAS.cDa Ecol\lacZ^ci-Dplac Ecol\lacZ^dpp.BS3.0 Ecol\lacZ^hh-P30 Ecol\lacZ^ptc.PG Scer\GAL4^ap-md544 Scer\GAL4^Bx-MS1096 Scer\GAL4^C-765 Scer\GAL4^GMR.PF Scer\GAL4^ptc-559.1 Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C} smo³ smo^{CK2SA.Scer\UAS.T:Hsap\MYC} smo^{CK2SA.tub.Scer\UAS.T:Hsap\MYC} smo^{PKA12.Scer\UAS} smo^{Scer\UAS.attB.T:Hsap\MYC} smo^{SD123CK2SD.Scer\UAS.T:Hsap\MYC} smo^{tub.Scer\UAS.T:Hsap\MYC} smo^{VK5.CK2SA.Scer\UAS.T:Hsap\MYC} Xlae\Gli1^{Scer\UAS.T:Hsap\MYC} Xlae\Gli2^{Scer\UAS.T:Hsap\MYC}
Constructs (22)
	P{BS3.0} P{GAL4-Act5C(FRT.CD2).P} P{GAL4-ninaE.GMR} P{GD8571} P{KK109263} P{ptcA-lacZ} P{UAS-ci.HA.-3P} P{UAS-ci.HA.W} P{UAS-CkIIα.FLAG} P{UAS-CkIIα.KM.FLAG} P{UAS-CkIIβ.FLAG} P{UAS-CkIIβ.RNAi.J} P{UAS-Dcr-2.D} P{UAS-GFP.U} P{UAS-Myc-Xlae\Gli1} P{UAS-Myc-Xlae\Gli2} P{UAS-smo.PKA12} PBac{UAS-smo.CK2SA.MYC} PBac{UAS-smo.Myc} PBac{UAS-smo.SD123CK2SD.MYC} PBac{UAS-smo.tub.CK2SA.MYC} PBac{UAS-tub-smo.MYC}
Genes (17)
	Avic\GFP ci CkIIα CkIIβ dbr Dcr-2 Ecol\lacZ en hh hyd rdx Scer\GAL4 smo T:Hsap\MYC T:Zzzz\FLAG Xlae\Gli1 Xlae\Gli2
Insertions (6)
	P{GawB}ap^md544 P{GawB}Bx^MS1096 P{GawB}C-765 P{GawB}ptc^559.1 P{lacW}ci^Dplac P{PZ}hh^P30
Natural transposons (2)
	P-element Tni\piggyBac