Reference Report

Reference
Citation	Vermehren-Schmaedick, A., Scudder, C., Timmermans, W., Morton, D.B. (2011). Drosophila gustatory preference behaviors require the atypical soluble guanylyl cyclases. J. Comp. Physiol. A, Neuroethol. Sens. Neural. Behav. Physiol. 197(7): 717--727. (Export to RIS)
FlyBase ID	FBrf0214366
Publication Type	Research paper
PubMed ID	21350862
PubMed Abstract	The intracellular messenger cGMP has been suggested to play a role in taste signal transduction in both vertebrates and invertebrates. In the present study, we have examined the role of the Drosophila atypical soluble guanylyl cyclases (sGCs), Gyc-89Da and Gyc-89Db, in larval and adult gustatory preference behaviors. We showed that in larvae, sucrose attraction requires Gyc-89Db and caffeine avoidance requires Gyc-89Da. In adult flies, sucrose attraction is unaffected by mutations in either gene whereas avoidance of low concentrations of caffeine is eliminated by loss of either gene. Similar defective behaviors were observed when cGMP increases were prevented by the expression of a cGMP-specific phosphodiesterase. We also showed that both genes were expressed in gustatory receptor neurons (GRNs) in larval and adult gustatory organs, primarily in a non-overlapping pattern, with the exception of a small group of cells in the adult labellum. In addition, in adults, several cells co-expressed the bitter taste receptor, Gr66a, with either Gyc-89Da or Gyc-89Db. We also showed that the electrophysiological responses of a GRN to caffeine were significantly reduced in flies mutant for the atypical sGCs, suggesting that at least part of the adult behavioral defects were due to a reduced ability to detect caffeine.
DOI	10.1007/s00359-011-0634-9
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Comp. Physiol. A, Neuroethol. Sens. Neural. Behav. Physiol.
Title	Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology
Publication Year	2001-
ISBN/ISSN	0340-7594 1432-1351
Data from Reference
Alleles (11)
	Avic\GFP^Gyc-89Da.1.9 Avic\GFP^Gyc-89Db.3.4 Btau\PDE5A^Scer\UAS.cBa Disc\RFP^Gr66a.PD Gyc88E^GD11230 Gyc-89Da^e01821 Gyc-89Da^Scer\UAS.cVa Gyc-89Db^MB03197 Gyc-89Db^Scer\UAS.cVa Scer\GAL4^Gyc-89Da.1.9 Scer\GAL4^Gyc-89Db.3.4
Constructs (9)
	P{GD11230} P{Gr66a-RFP.D} P{Gyc-89Da-GAL4.1.9} P{Gyc-89Da-GFP.1.9} P{Gyc-89Db-GAL4.3.4} P{Gyc-89Db-GFP.3.4} P{UAS-Gyc-89Da.V} P{UAS-Gyc-89Db.V} P{UAS-PDE5A.B}
Genes (8)
	Avic\GFP Btau\PDE5A Disc\RFP Gr66a Gyc88E Gyc-89Da Gyc-89Db Scer\GAL4
Insertions (2)
	Mi{ET1}Gyc-89Db^MB03197 PBac{RB}Gyc-89Da^e01821
Transcripts (4)
	Avic\GFP^Gyc-89Db.3.4RA Disc\RFP^Gr66a.PDRA Scer\GAL4^Gyc-89Da.1.9RA Scer\GAL4^Gyc-89Db.3.4RA