Reference Report

Reference
Citation	Moon, S., Cho, B., Min, S.H., Lee, D., Chung, Y.D. (2011). The THO complex is required for nucleolar integrity in Drosophila spermatocytes. Development 138(17): 3835--3845. (Export to RIS)
FlyBase ID	FBrf0214600
Publication Type	Research paper
PubMed ID	21828100
PubMed Abstract	The THO complex is a conserved multisubunit protein complex that functions in the formation of export-competent messenger ribonucleoprotein (mRNP). Although the complex has been studied extensively at the single-cell level, its exact role at the multicellular organism level has been poorly understood. Here, we isolated a novel Drosophila male sterile mutant, garmcho (garm). Positional cloning indicated that garm encodes a subunit of the Drosophila THO complex, THOC5. Flies lacking THOC5 showed a meiotic arrest phenotype with severe nucleolar disruption in primary spermatocytes. A functional GFP-tagged fusion protein, THOC5-GFP, revealed a unique pattern of THOC5 localization near the nucleolus. The nucleolar distribution of a testis-specific TATA binding protein (TBP)-associated factor (tTAF), SA, which is required for the expression of genes responsible for sperm differentiation, was severely disrupted in mutant testes lacking THOC5. But THOC5 appeared to be largely dispensable for the expression and nuclear export of either tTAF target mRNAs or tTAF-independent mRNAs. Taken together, our study suggests that the Drosophila THO complex is necessary for proper spermatogenesis by contribution to the establishment or maintenance of nucleolar integrity rather than by nuclear mRNA export in spermatocytes.
DOI	10.1242/dev.056945
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Secondary IDs
Language of Publication	English
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Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Aberrations (3)
	Df(2R)5MR2 Df(2R)5MR4 Df(2R)5MR12
Alleles (25)
	alpha-catenin-related^tBRV CG2970^t7.9 CG2970^tH3 CG3803^t7.9 CG3803^tH3 CG16787^t7.9 CG16787^tBRV Fmo-1^tBRV fzr2^t7.9 fzr2^tH3 Gαs^tH3 mia¹ Mmus\Thoc5^Scer\UAS.cMa Nopp140^{RGG.hs.T:Avic\GFP} Pc^{T:Avic\GFP-EGFP} sa¹ sa^{T:Avic\GFP-EGFP} Scer\GAL4^da.G32 T:Avic\GFP^EGFP thoc5¹ thoc5^+t7.9 thoc5^e00906 thoc5^{T:Avic\GFP-EGFP} thoc6^e00298 thoc7^d05792
Constructs (9)
	P{BRV} P{GAL4-da.G32} P{H3} P{hs-Nopp140.RGG.GFP} P{Pc^{T:Avic\GFP-EGFP}} P{sa-EGFP} P{thoc5.eGFP} P{thoc5^+t7.9} P{UAS-mThoc5}
Genes (26)
	alpha-catenin-related Avic\GFP bol CG2970 CG3803 CG16787 CycB dj Fib Fmo-1 fzo fzr2 Gαs Hpr1 mia Mmus\Thoc5 Mst87F Nopp140 Pc sa Scer\GAL4 tho2 thoc5 thoc6 thoc7 twe
Insertions (5)
	P{EP}CG3065^EP316 P{EP}Tal^EP489 P{XP}thoc7^d05792 PBac{RB}thoc5^e00906 PBac{RB}thoc6^e00298
Natural transposons (1)
	P-element