Reference Report

Reference
Citation	Becam, I., Rafel, N., Hong, X., Cohen, S.M., Milán, M. (2011). Notch-mediated repression of bantam miRNA contributes to boundary formation in the Drosophila wing. Development 138(17): 3781--3789. (Export to RIS)
FlyBase ID	FBrf0214603
Publication Type	Research paper
PubMed ID	21795284
PubMed Abstract	Subdivision of proliferating tissues into adjacent compartments that do not mix plays a key role in animal development. The Actin cytoskeleton has recently been shown to mediate cell sorting at compartment boundaries, and reduced cell proliferation in boundary cells has been proposed as a way of stabilizing compartment boundaries. Cell interactions mediated by the receptor Notch have been implicated in the specification of compartment boundaries in vertebrates and in Drosophila, but the molecular effectors remain largely unidentified. Here, we present evidence that Notch mediates boundary formation in the Drosophila wing in part through repression of bantam miRNA. bantam induces cell proliferation and we have identified the Actin regulator Enabled as a new target of bantam. Increased levels of Enabled and reduced proliferation rates contribute to the maintenance of the dorsal-ventral affinity boundary. The activity of Notch also defines, through the homeobox-containing gene cut, a distinct population of boundary cells at the dorsal-ventral (DV) interface that helps to segregate boundary from non-boundary cells and contributes to the maintenance of the DV affinity boundary.
DOI	10.1242/dev.064774
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Secondary IDs
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Aberrations (1)
	Df(3L)ban^Δ1
Alleles (30)
	Avic\GFP^{EGFP.ban.αTub84B} Avic\GFP^{EGFP.Del.tub.ena3'UTR} Avic\GFP^{EGFP.Mut.tub.ena3'UTR} Avic\GFP^{EGFP.tub.ena3'UTR} ban^{C.Scer\UAS.T:Avic\GFP-EGFP} ct⁶ ct^C145 ct^GD1237 ct^Scer\UAS.cPa CycE^Scer\UAS.cLa Dcr-1^GD11429 Disc\RFP^{dsRed.Scer\UAS.ban-sponge} dm^GD1419 dm^Scer\UAS.cZa Ecol\lacZ^ap-rK568 Ecol\lacZ^ct.wHZ Ecol\lacZ^Dr-lacZ-Δ89 Ecol\lacZ^trn-S064117 ena^GD8910 ena^KK107752 N^{dsRNA.P.Scer\UAS} N^l1N-ts1 Scer\GAL4^ap-md544 Scer\GAL4^salm-459.2 Scer\GAL4^sd-SG29.1 Scer\GAL4^vg.int2.1 sqh^{RLC.T:Avic\GFP-S65T} stg^Scer\UAS.cNa trbl^Scer\UAS.cMa Zzzz\actA^{FP4mito.Scer\UAS.T:Avic\GFP-EGFP}
Constructs (20)
	P{ctwHZ} P{GD1237} P{GD1419} P{GD8910} P{GD11429} P{KK107752} P{sqh-GFP.RLC} P{Tub-EGFP.ban} P{tub-eGFP.ena-3'UTR.Mut} P{tub-eGFP.ena-3'UTR} P{UAS-C} P{UAS-ct.P} P{UAS-CycE.L} P{UAS-dm.Z} P{UAS-dsRed.ban-sponge} P{UAS-GFP-FP4mito} P{UAS-N.dsRNA.P} P{UAS-stg.N} P{UAS-trbl.M} P{vg(int2.1)-GAL4}
Genes (20)
	Avic\GFP ban caps ci ct CycE Dcr-1 Disc\RFP dm Ecol\lacZ ena N Scer\GAL4 sens sqh stg trbl trn wg Zzzz\actA
Insertions (6)
	P{5'lacZ}Dr^lacZ-Δ89 P{GawB}459.2 P{GawB}ap^md544 P{GawB}sd^SG29.1 P{lacW}trn^S064117 P{PZ}ap^rK568
Natural transposons (1)
	P-element