Reference Report

Reference
Citation	Seisenbacher, G., Hafen, E., Stocker, H. (2011). MK2-Dependent p38b Signalling Protects Drosophila Hindgut Enterocytes against JNK-Induced Apoptosis under Chronic Stress. PLoS Genet. 7(8): e1002168. (Export to RIS)
FlyBase ID	FBrf0214607
Publication Type	Research paper
PubMed ID	21829386
PubMed Abstract	The integrity of the intestinal epithelium is crucial for the barrier function of the gut. Replenishment of the gut epithelium by intestinal stem cells contributes to gut homeostasis, but how the differentiated enterocytes are protected against stressors is less well understood. Here we use the Drosophila larval hindgut as a model system in which damaged enterocytes are not replaced by stem cell descendants. By performing a thorough genetic analysis, we demonstrate that a signalling complex consisting of p38b and MK2 forms a branch of SAPK signalling that is required in the larval hindgut to prevent stress-dependent damage to the enterocytes. Impaired p38b/MK2 signalling leads to apoptosis of the enterocytes and a subsequent loss of hindgut epithelial integrity, as manifested by the deterioration of the overlaying muscle layer. Damaged hindguts show increased JNK activity, and removing upstream activators of JNK suppresses the loss of hindgut homeostasis. Thus, the p38/MK2 complex ensures homeostasis of the hindgut epithelium by counteracting JNK-mediated apoptosis of the enterocytes upon chronic stress.
DOI	10.1371/journal.pgen.1002168
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Language of Publication	English
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Publication Type	Journal
Abbreviation	PLoS Genet.
Title	PLoS Genetics
Publication Year	2005-
ISBN/ISSN	1553-7404 1553-7390
Data from Reference
Alleles (39)
	Avic\GFP^Nrg-G00305 Avic\GFP^Scer\UAS.cUa bsk^{K53R.Scer\UAS} Ecol\lacZ^msn-06946 Ecol\lacZ^puc-E69 lic^d13 MAPk-Ak2^+tSa MAPk-Ak2^GE3296 MAPk-Ak2^{K49A.Scer\UAS} MAPk-Ak2^Scer\UAS.cSa MAPk-Ak2^T:Avic\GFP MAPk-Ak2^Δ1A MAPk-Ak2^Δ12 MAPk-Ak2^Δ38 MAPk-Ak2^Δ41 MAPk-Ak2^Δ43 Mekk1^Ur36 Mkk4^G414 Mkk4^G587 Mmus\Bax^Scer\UAS.cGb Mmus\Mapkapk2^Scer\UAS.cSa Mpk2¹ Mpk2^{DPTD.Scer\UAS.T:Sjap\GST} Mpk2^{Scer\UAS.T:Sjap\GST} p38b^{AGF.Scer\UAS.T:Sjap\GST} p38b^d27 p38b^{KR.Scer\UAS.T:Sjap\GST} p38b^{Scer\UAS.EPSV.T:Sjap\GST} p38b^{Scer\UAS.T:Sjap\GST} Scer\GAL4^Act.PU Scer\GAL4^byn.n2 Scer\GAL4^cad-em459 Scer\GAL4^da.G32 Scer\GAL4^en-e16E Scer\GAL4^He.PZ Scer\GAL4^{Myo31DF-NP0001} Scer\GAL4^puc-GAL4E69 Tak1² Tak1⁴
Constructs (19)
	P{Act-GAL4.U} P{byn-GAL4.n2} P{EP} P{GAL4-da.G32} P{He-GAL4.Z} P{MAPk-Ak2^+tSa} P{MAPk-Ak2-GFP} P{UAS-bax.G} P{UAS-bsk.K53R} P{UAS-GFP.U} P{UAS-MAPk-Ak2.K49A} P{UAS-MAPk-Ak2.S} P{UAS-Mapkapk2.S} P{UAS-Mpk2.DPTD.GST} P{UAS-Mpk2.GST} P{UAS-p38b.AGF.GST} P{UAS-p38b.EPSV.GST} P{UAS-p38b.GST} P{UAS-p38b.KR.GST}
Genes (17)
	Avic\GFP bsk Ecol\lacZ lic MAPk-Ak2 Mekk1 Mkk4 Mmus\Bax Mmus\Mapkapk2 Mpk2 msn Nrg p38b puc Scer\GAL4 T:Sjap\GST Tak1
Insertions (8)
	P{A92}puc^E69 P{en2.4-GAL4}e16E P{EP}MAPk-Ak2^GE3296 P{GAL4E69}puc^GAL4E69 P{GawB}cad^em459 P{GawB}Myo31DF^NP0001 P{PTT-GA}Nrg^G00305 P{PZ}msn⁰⁶⁹⁴⁶
Natural transposons (1)
	P-element