Reference Report

Reference
Citation	Callejo, A., Bilioni, A., Mollica, E., Gorfinkiel, N., Andrés, G., Ibáñez, C., Torroja, C., Doglio, L., Sierra, J., Guerrero, I. (2011). Feature Article: From the Cover: Dispatched mediates Hedgehog basolateral release to form the long-range morphogenetic gradient in the Drosophila wing disk epithelium. Proc. Natl. Acad. Sci. U.S.A. 108(31): 12591--12598. (Export to RIS)
FlyBase ID	FBrf0214615
Publication Type	Research paper
PubMed ID	21690386
PubMed Abstract	Hedgehog (Hh) moves from the producing cells to regulate the growth and development of distant cells in a variety of tissues. Here, we have investigated the mechanism of Hh release from the producing cells to form a morphogenetic gradient in the Drosophila wing imaginal disk epithelium. We describe that Hh reaches both apical and basolateral plasma membranes, but the apical Hh is subsequently internalized in the producing cells and routed to the basolateral surface, where Hh is released to form a long-range gradient. Functional analysis of the 12-transmembrane protein Dispatched, the glypican Dally-like (Dlp) protein, and the Ig-like and FNNIII domains of protein Interference Hh (Ihog) revealed that Dispatched could be involved in the regulation of vesicular trafficking necessary for basolateral release of Hh, Dlp, and Ihog. We also show that Dlp is needed in Hh-producing cells to allow for Hh release and that Ihog, which has been previously described as an Hh coreceptor, anchors Hh to the basolateral part of the disk epithelium.
DOI	10.1073/pnas.1106881108
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Proc. Natl. Acad. Sci. U.S.A.
Title	Proceedings of the National Academy of Sciences of the United States of America
Publication Year	1915-
ISBN/ISSN	0027-8424
Data from Reference
Alleles (25)
	ci^Scer\UAS.cAa disp^{AAA.Scer\UAS.T:Disc\RFP} disp^{Scer\UAS.T:Avic\GFP-YFP} disp^{Scer\UAS.T:Ivir\HA1} dlp^MH20 dlp^{Scer\UAS.T:SS-wg,T:Avic\GFP} hh^{C85S.N.Scer\UAS.T:Avic\GFP} hh^{C85S.Scer\UAS.T:Avic\GFP} hh^{N.Scer\UAS.T:Avic\GFP} hh^{Scer\UAS.T:Avic\GFP-EGFP} ihog^Scer\UAS.cZa ihog^{Scer\UAS.T:Avic\GFP-YFP.Venus} ihog^{Scer\UAS.T:Disc\RFP} Rab8^{T22N.Scer\UAS.P\T.T:Avic\GFP-YFP} Scer\GAL4^AB1 Scer\GAL4^ap-md544 Scer\GAL4^en.PU Scer\GAL4^hh-Gal4 Scer\GAL4^ptc-559.1 Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C} Scer\GAL80^ts.αTub84B shi¹ shi^{K44A.Scer\UAS} T:Avic\GFP^YFP.Venus T:Avic\GFP^YFP
Constructs (17)
	P{en-GAL4.U} P{GAL4-Act5C(FRT.CD2).P} P{tubP-GAL80^ts} P{UAS-ci.A} P{UAS-disp.HA1} P{UAS-disp^AAA-RFP} P{UAS-GFP::dlp} P{UAS-hh.C85S.GFP} P{UAS-hh.C85S.N.GFP} P{UAS-hh.GFP} P{UAS-hh.N.GFP} P{UAS-ihog.RFP} P{UAS-ihog.venus} P{UAS-ihog.Z} P{UASp-YFP.Rab8.T22N} P{UAS-shi.K44A} P{UAS-YFP-disp}
Genes (17)
	ci disp dlp dor dpp en hh ihog kn ptc Rab4 Rab5 Rab8 Scer\GAL4 Scer\GAL80 shi T:Disc\RFP
Insertions (4)
	P{GAL4}hh^Gal4 P{GawB}AB1 P{GawB}ap^md544 P{GawB}ptc^559.1
Natural transposons (1)
	P-element