Reference Report

Reference
Citation	Duan, H., Dai, Q., Kavaler, J., Bejarano, F., Medranda, G., Nègre, N., Lai, E.C. (2011). Insensitive is a corepressor for Suppressor of Hairless and regulates Notch signalling during neural development. EMBO J. 30(15): 3120--3133. (Export to RIS)
FlyBase ID	FBrf0214638
Publication Type	Research paper
PubMed ID	21765394
PubMed Abstract	The Notch intracellular domain functions as a co-activator for the DNA-binding protein Suppressor of Hairless (Su(H)) to mediate myriad cell fate decisions. Notch pathway activity is balanced by transcriptional repression, mediated by Su(H) in concert with its Drosophila corepressor Hairless. We demonstrate that the Drosophila neural BEN-solo protein Insensitive (Insv) is a nuclear factor that inhibits Notch signalling during multiple peripheral nervous system cell fate decisions. Endogenous Insv was particularly critical when repressor activity of Su(H) was compromised. Reciprocally, ectopic Insv generated several Notch loss-of-function phenotypes, repressed most Notch targets in the E(spl)-C, and opposed Notch-mediated activation of an E(spl)m3-luc reporter. A direct role for Insv in transcriptional repression was indicated by binding of Insv to Su(H), and by strong chromatin immunoprecipitation of endogenous Insv to most E(spl)-C loci. Strikingly, ectopic Insv fully rescued sensory organ precursors in Hairless null clones, indicating that Insv can antagonize Notch independently of Hairless. These data shed first light on the in vivo function for a BEN-solo protein as an Su(H) corepressor in the Notch pathway regulating neural development.
DOI	10.1038/emboj.2011.218
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Language of Publication	English
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Publication Type	Journal
Abbreviation	EMBO J.
Title	The EMBO Journal
Publication Year	1982-
ISBN/ISSN	0261-4189
Data from Reference
Alleles (18)
	H¹ H² H^E31 insv^23B insv^23I insv^+t3.67 insv^Scer\UAS.cDa insv^unspecified N^l1N-ts1 numb^hs.PU Scer\GAL4^Eq1 Scer\GAL4^hs.PB Scer\GAL4^ptc-559.1 Scer\GAL4^sca.PU Scer\GAL4^tub.PU Scer\GAL4^vg.PU Su(H)^{Scer\UAS.T:Hsap\MYC} w^+mW.hs
Constructs (8)
	P{GAL4-Hsp70.PB} P{hs-numb} P{insv.+3.67} P{sca-GAL4.U} P{tub-GAL4.U} P{UAS-insv.D} P{UAS-Su(H).myc} P{vg-GAL4.U}
Genes (28)
	CG12290 CG34245 ct Cyp309a2 E(spl)m2-BFM E(spl)m3-HLH E(spl)m4-BFM E(spl)m5-HLH E(spl)m6-BFM E(spl)m7-HLH E(spl)m8-HLH E(spl)mα-BFM E(spl)mβ-HLH E(spl)mγ-HLH E(spl)mδ-HLH elav H insv Kaz-m1 N numb peb pros Scer\GAL4 sens Su(H) sv w
Insertions (2)
	P{GAL4-Hsp70.PB}l(3)Eq1^Eq1 P{GawB}ptc^559.1
Natural transposons (1)
	P-element