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Rose, P.P., Hanna, S.L., Spiridigliozzi, A., Wannissorn, N., Beiting, D.P., Ross, S.R., Hardy, R.W., Bambina, S.A., Heise, M.T., Cherry, S. (2011). Natural resistance-associated macrophage protein is a cellular receptor for sindbis virus in both insect and Mammalian hosts.  Cell Host Microbe 10(2): 97--104.
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Publication Type
Research paper

Alphaviruses, including several emerging human pathogens, are a large family of mosquito-borne viruses with Sindbis virus being a prototypical member of the genus. The host factor requirements and receptors for entry of this class of viruses remain obscure. Using a Drosophila system, we identified the divalent metal ion transporter natural resistance-associated macrophage protein (NRAMP) as a host cell surface molecule required for Sindbis virus binding and entry into Drosophila cells. Consequently, flies mutant for dNRAMP were protected from virus infection. NRAMP2, the ubiquitously expressed vertebrate homolog, mediated binding and infection of Sindbis virus into mammalian cells, and murine cells deficient for NRAMP2 were nonpermissive to infection. Alphavirus glycoprotein chimeras demonstrated that the requirement for NRAMP2 is at the level of Sindbis virus entry. Given the conserved structure of alphavirus glycoproteins, and the widespread use of transporters for viral entry, other alphaviruses may use conserved multipass membrane proteins for infection.

PubMed ID
PubMed Central ID
PMC3164510 (PMC) (EuropePMC)
Related Publication(s)

Alphavirus Entry: NRAMP Leads the Way.
Stiles and Kielian, 2011, Cell Host Microbe 10(2): 92--93 [FBrf0214644]

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    Language of Publication
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Cell Host Microbe
    Cell Host & Microbe
    Publication Year
    1931-3128 1934-6069
    Data From Reference
    Alleles (1)
    Genes (2)
    Human Disease Models (1)
    Cell Lines (2)
    Insertions (1)