Reference Report

Reference
Citation	Lee, P.T., Lin, H.W., Chang, Y.H., Fu, T.F., Dubnau, J., Hirsh, J., Lee, T., Chiang, A.S. (2011). Serotonin-mushroom body circuit modulating the formation of anesthesia-resistant memory in Drosophila. Proc. Natl. Acad. Sci. U.S.A. 108(33): 13794--13799. (Export to RIS)
FlyBase ID	FBrf0214737
Publication Type	Research paper
PubMed ID	21808003
PubMed Abstract	Pavlovian olfactory learning in Drosophila produces two genetically distinct forms of intermediate-term memories: anesthesia-sensitive memory, which requires the amnesiac gene, and anesthesia-resistant memory (ARM), which requires the radish gene. Here, we report that ARM is specifically enhanced or inhibited in flies with elevated or reduced serotonin (5HT) levels, respectively. The requirement for 5HT was additive with the memory defect of the amnesiac mutation but was occluded by the radish mutation. This result suggests that 5HT and Radish protein act on the same pathway for ARM formation. Three supporting lines of evidence indicate that ARM formation requires 5HT released from only two dorsal paired medial (DPM) neurons onto the mushroom bodies (MBs), the olfactory learning and memory center in Drosophila: (i) DPM neurons were 5HT-antibody immunopositive; (ii) temporal inhibition of 5HT synthesis or release from DPM neurons, but not from other serotonergic neurons, impaired ARM formation; (iii) knocking down the expression of d5HT1A serotonin receptors in α/β MB neurons, which are innervated by DPM neurons, inhibited ARM formation. Thus, in addition to the Amnesiac peptide required for anesthesia-sensitive memory formation, the two DPM neurons also release 5HT acting on MB neurons for ARM formation.
DOI	10.1073/pnas.1019483108
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Proc. Natl. Acad. Sci. U.S.A.
Title	Proceedings of the National Academy of Sciences of the United States of America
Publication Year	1915-
ISBN/ISSN	0027-8424
Data from Reference
Alleles (28)
	5-HT1A^KK108407 5-HT1B^GD17040 amn^X8 Avic\GFP^{Scer\UAS.T:SV40\nls2} Dcr-1^Scer\UAS.cDa Ddc^GD1189 Ecol\lexA^L0111 Hn^GD12227 Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} ple^GD1181 rad¹ Rnor\CD2^{Ecol\lexAop.T:Avic\GFP} Scer\GAL4⁵⁰¹⁵ Scer\GAL4¹³¹⁰⁹ Scer\GAL4^c305a Scer\GAL4^c316 Scer\GAL4^c739 Scer\GAL4^Ddc.PL Scer\GAL4^elav-C155 Scer\GAL4^ey-OK107 Scer\GAL4^Hn.247 Scer\GAL4^Hn.493 Scer\GAL4^Hn.819 Scer\GAL4^Hn.996 Scer\GAL4^ple.PF Scer\GAL4^VT64246 Scer\GAL80^ts.αTub84B shi^1.Scer\UAS
Constructs (16)
	P{Ddc-GAL4.L} P{GD1181} P{GD1189} P{GD12227} P{Hn.247-Gal4} P{Hn.493-Gal4} P{Hn.819-Gal4} P{Hn.996-Gal4} P{KK108407} P{lexAop-rCD2-GFP} P{ple-GAL4.F} P{tubP-GAL80^ts} P{UAS-Dcr-1.D} P{UAS-GFP.nls} P{UAS-mCD8::GFP.L} P{UAS-shi^ts1.K}
Genes (14)
	5-HT1A 5-HT1B amn Avic\GFP Dcr-1 Ddc Hn Mmus\Cd8a ple rad Rnor\CD2 Scer\GAL4 Scer\GAL80 shi
Insertions (8)
	P{GawB}5015 P{GawB}c305a P{GawB}c316 P{GawB}elav^C155 P{GawB}ey^OK107 P{GawB}Hr39^c739 P{GT1}BG00015 P{UAS-lexA-GAD}L0111
Natural transposons (1)
	P-element
Transcripts (4)
	Ecol\lexA^L0111RA Scer\GAL4^c316RA Scer\GAL4^c739RA Scer\GAL4^VT64246RA