Reference Report

Reference
Citation	Zamparini, A.L., Davis, M.Y., Malone, C.D., Vieira, E., Zavadil, J., Sachidanandam, R., Hannon, G.J., Lehmann, R. (2011). Vreteno, a gonad-specific protein, is essential for germline development and primary piRNA biogenesis in Drosophila. Development 138(18): 4039--4050. (Export to RIS)
FlyBase ID	FBrf0214783
Publication Type	Research paper
PubMed ID	21831924
PubMed Abstract	In Drosophila, Piwi proteins associate with Piwi-interacting RNAs (piRNAs) and protect the germline genome by silencing mobile genetic elements. This defense system acts in germline and gonadal somatic tissue to preserve germline development. Genetic control for these silencing pathways varies greatly between tissues of the gonad. Here, we identified Vreteno (Vret), a novel gonad-specific protein essential for germline development. Vret is required for piRNA-based transposon regulation in both germline and somatic gonadal tissues. We show that Vret, which contains Tudor domains, associates physically with Piwi and Aubergine (Aub), stabilizing these proteins via a gonad-specific mechanism that is absent in other fly tissues. In the absence of vret, Piwi-bound piRNAs are lost without changes in piRNA precursor transcript production, supporting a role for Vret in primary piRNA biogenesis. In the germline, piRNAs can engage in an Aub- and Argonaute 3 (AGO3)-dependent amplification in the absence of Vret, suggesting that Vret function can distinguish between primary piRNAs loaded into Piwi-Aub complexes and piRNAs engaged in the amplification cycle. We propose that Vret plays an essential role in transposon regulation at an early stage of primary piRNA processing.
DOI	10.1242/dev.069187
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Secondary IDs
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Aberrations (1)
	Df(3R)Exel6192
Alleles (25)
	aub^HN2 aub^QC42 aub^{Scer\UAS.P\T.T:Avic\GFP-m6} Ecol\lacZ^gypsy.p12 flam^BG02658 flam^KG00476 piwi¹ piwi² piwi^{Scer\UAS.P\T.T:Ivir\HA1} Scer\GAL4^Act5C.PU Scer\GAL4^ap-md544 Scer\GAL4^bab1-Pgal4-2 Scer\GAL4^C587 Scer\GAL4^{nos.UTR.T:Hsim\VP16} Scer\GAL4^otu.PR Scer\GAL4^tj-NP1624 vret^1B vret¹⁵ vret³⁹ vret⁴⁶ vret⁴⁹ vret⁷⁰ vret^148-15 vret^148-60 vret^{Scer\UAS.P\T.T:Hsap\MYC}
Constructs (7)
	P{Act5C-GAL4.U} P{GAL4::VP16-nos.UTR} P{gypsy-lacZ.p12} P{otu-GAL4.R} P{UASp-GFP-aub.H} P{UASp-piwi.HA} P{UASp-vret.myc}
Genes (13)
	AGO3 armi aub Ecol\lacZ flam piRNA piwi Scer\GAL4 T:Hsap\MYC T:Ivir\HA1 tj vas vret
Insertions (4)
	P{GAL4}C587 P{GawB}ap^md544 P{GawB}bab1^Pgal4-2 P{GawB}NP1624
Natural transposons (10)
	GATE gtwin gypsy gypsy5 Idefix mdg1 P-element roo Tabor ZAM