Reference Report

Reference
Citation	Ayyub, C. (2011). Cullin-5 and cullin-2 play a role in the development of neuromuscular junction and the female germ line of Drosophila. J. Genet. 90(2): 239--249. (Export to RIS)
FlyBase ID	FBrf0214802
Publication Type	Research paper
PubMed ID	21869472
PubMed Abstract	Cullins confer substrate specificity to E3-ligases which are multi-protein complexes involved in ubiquitin-mediated protein degradation or modification. There are six cullin genes in Drosophila melanogaster. We have raised an antibody against Cul-5 and demonstrated that it expresses in neuronal and non-neuronal cells throughout development. In the embryonic tracheal system, Cul-5 is enriched at fusion sites together with E-Cadherin and Fasciclin III. Mutations of cul-5 do not affect tracheal development but do show defects in the organization of synaptic boutons at the larval neuromuscular junction where the protein is expressed in a subset of motoneuron terminals. Loss of function of another cullin gene 'cul-2' results in similar defects at the larval neuromuscular junction although cul-2;cul-5 double mutants do not show an enhanced phenotype. Both cul-2 and cul-5 mutants show similar aberrations in the development of female germ line. Our results suggest that both of these cullin proteins participate in similar developmental processes.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Genet.
Title	Journal of Genetics
Publication Year	1910-
ISBN/ISSN	0022-1333
Data from Reference
Aberrations (1)
	Df(3R)3450
Alleles (8)
	Cul-2⁰²⁰⁷⁴ Cul-2^EY09124 Cul-2^GD8913 Cul-5^exB Cul-5^EY00051 Cul-5^EY21463 Ecol\lacZ^Cul-2-02074 Scer\GAL4^elav.PU
Constructs (2)
	P{elav-GAL4.U} P{GD8913}
Genes (12)
	CG11261 Cul-2 Cul-3 Cul-4 Cul-5 dlg1 Ecol\lacZ Fas2 Fas3 lin19 Scer\GAL4 shg
Insertions (5)
	P{EPgy2}Cul-2^EY09124 P{EPgy2}Cul-5^EY00051 P{EPgy2}Cul-5^EY21463 P{GD8913}v19297 P{PZ}Cul-2⁰²⁰⁷⁴
Transcripts (1)
	Cul-2⁰²⁰⁷⁴RA