Reference Report

Reference
Citation	Truscott, M., Islam, A.B., López-Bigas, N., Frolov, M.V. (2011). mir-11 limits the proapoptotic function of its host gene, dE2f1. Genes Dev. 25(17): 1820--1834. (Export to RIS)
FlyBase ID	FBrf0215211
Publication Type	Research paper
PubMed ID	21856777
PubMed Abstract	The E2F family of transcription factors regulates the expression of both genes associated with cell proliferation and genes that regulate cell death. The net outcome is dependent on cellular context and tissue environment. The mir-11 gene is located in the last intron of the Drosophila E2F1 homolog gene dE2f1, and its expression parallels that of dE2f1. Here, we investigated the role of miR-11 and found that miR-11 specifically modulated the proapoptotic function of its host gene, dE2f1. A mir-11 mutant was highly sensitive to dE2F1-dependent, DNA damage-induced apoptosis. Consistently, coexpression of miR-11 in transgenic animals suppressed dE2F1-induced apoptosis in multiple tissues, while exerting no effect on dE2F1-driven cell proliferation. Importantly, miR-11 repressed the expression of the proapoptotic genes reaper (rpr) and head involution defective (hid), which are directly regulated by dE2F1 upon DNA damage. In addition to rpr and hid, we identified a novel set of cell death genes that was also directly regulated by dE2F1 and miR-11. Thus, our data support a model in which the coexpression of miR-11 limits the proapoptotic function of its host gene, dE2f1, upon DNA damage by directly modulating a dE2F1-dependent apoptotic transcriptional program.
DOI	10.1101/gad.16947411
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genes Dev.
Title	Genes & Development
Publication Year	1987-
ISBN/ISSN	0890-9369
Data from Reference
Alleles (15)
	BacA\p35^Scer\UAS.cUa Cele\ced-3^Scer\UAS.cSa Dp^Scer\UAS.cDa E2f⁹¹ E2f⁰⁷¹⁷² E2f^rM729 E2f^Scer\UAS.cNa E2f^Δ1 mir-11^{Scer\UAS.T:Disc\RFP-DsRed2} mir-11^Δ1 rpr^GMR.PW Scer\GAL4^Act88F.PD Scer\GAL4^GMR.PF W^GMR.PG yki^{S168A.Scer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1}
Constructs (10)
	P{Act88F-GAL4.D} P{GAL4-ninaE.GMR} P{GMR-hid} P{GMR-rpr.W} P{UAS-Cele\ced-3.S} P{UAS-Dp.D} P{UAS-DsRed2-mir-11} P{UAS-E2f.N} P{UAS-p35.U} P{UAS-yki.S168A.GFP.HA}
Genes (17)
	aop BacA\p35 cbt Cele\ced-3 Dp E2f Eip93F grim mats mir-11 Pink1 rpr Scer\GAL4 scyl skl W yki
Insertions (2)
	P{XP}E2f^d01508 PBac{RB}InR^e01952