Reference Report

Reference
Citation	Cairrao, F., Halees, A.S., Khabar, K.S., Morello, D., Vanzo, N. (2009). AU-rich elements regulate Drosophila gene expression. Mol. Cell. Biol. 29(10): 2636--2643. (Export to RIS)
FlyBase ID	FBrf0215319
Publication Type	Research paper
PubMed ID	19273595
PubMed Abstract	In mammals, AU-rich elements (AREs) are critical regulators of mRNA turnover. They recruit ARE-binding proteins that inhibit or stimulate rapid mRNA degradation in response to stress or developmental cues. Using a bioinformatics approach, we have identified AREs in Drosophila melanogaster 3' untranslated regions and validated their cross-species conservation in distant Drosophila genomes. We have generated a Drosophila ARE database (D-ARED) and established that about 16% of D. melanogaster genes contain the mammalian ARE signature, an AUUUA pentamer in an A/U-rich context. Using candidate ARE genes, we show that Drosophila AREs stimulate reporter mRNA decay in cultured cells and in the physiological context of the immune response in D. melanogaster. In addition, we found that the conserved ARE-binding protein Tis11 regulates temporal gene expression through ARE-mediated decay (AMD) in D. melanogaster. Our work reveals that AREs are conserved and functional cis regulators of mRNA decay in Drosophila and highlights this organism as a novel model system to unravel in vivo the contribution of AMD to various processes.
DOI	10.1128/MCB.01506-08
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Mol. Cell. Biol.
Title	Molecular and Cellular Biology
Publication Year	1981-
ISBN/ISSN	0270-7306
Data from Reference
Alleles (3)
	Ppyr\LUC^{CecA1.3'UTR.CecA1.AREmut} Ppyr\LUC^{CecA1.3'UTR.CecA1.WT} Scer\GAL4^Cg.PA
Constructs (3)
	M{CecA1-LUC.3'UTR.CecA1.AREmut} M{CecA1-LUC.3'UTR.CecA1.WT} P{Cg-GAL4.A}
Genes (6)
	arm bnl CecA1 Ppyr\LUC Scer\GAL4 Tis11
Natural transposons (1)
	Dmau\mariner