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Ma, X., Lynch, H.E., Scully, P.C., Hutson, M.S. (2009). Probing embryonic tissue mechanics with laser hole drilling.  Phys. Biol. 6(3): 036004.
FlyBase ID
FBrf0215379
Publication Type
Research paper
Abstract
We use laser hole drilling to assess the mechanics of an embryonic epithelium during development-in vivo and with subcellular resolution. We ablate a subcellular cylindrical hole clean through the epithelium and track the subsequent recoil of adjacent cells (on ms time scales). We investigate dorsal closure in the fruit fly with emphasis on apical constriction of amnioserosa cells. The mechanical behavior of this epithelium falls between that of a continuous sheet and a 2D cellular foam (a network of tensile interfaces). Tensile stress is carried both by cell-cell interfaces and by the cells' apical actin networks. Our results show that stress is slightly concentrated along interfaces (1.6-fold), but only in early closure. Furthermore, closure is marked by a decrease in the recoil power-law exponent, implying a transition to a more solid-like tissue. We use the site and stage dependence of the recoil kinetics to constrain how the cellular mechanics change during closure. We apply these results to test extant computational models.
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Phys. Biol.
    Title
    Physical Biology
    Publication Year
    2004
    ISBN/ISSN
    1478-3967 1478-3975
    Data From Reference
    Genes (2)