Reference Report

Reference
Citation	Wang, C., Li, S., Januschke, J., Rossi, F., Izumi, Y., Garcia-Alvarez, G., Gwee, S.S., Soon, S.B., Sidhu, H.K., Yu, F., Matsuzaki, F., Gonzalez, C., Wang, H. (2011). An ana2/ctp/mud complex regulates spindle orientation in Drosophila neuroblasts. Dev. Cell 21(3): 520--533. (Export to RIS)
FlyBase ID	FBrf0215580
Publication Type	Research paper
PubMed ID	21920316
PubMed Abstract	Drosophila neural stem cells, larval brain neuroblasts (NBs), align their mitotic spindles along the apical/basal axis during asymmetric cell division (ACD) to maintain the balance of self-renewal and differentiation. Here, we identified a protein complex composed of the tumor suppressor anastral spindle 2 (Ana2), a dynein light-chain protein Cut up (Ctp), and Mushroom body defect (Mud), which regulates mitotic spindle orientation. We isolated two ana2 alleles that displayed spindle misorientation and NB overgrowth phenotypes in larval brains. The centriolar protein Ana2 anchors Ctp to centrioles during ACD. The centriolar localization of Ctp is important for spindle orientation. Ana2 and Ctp localize Mud to the centrosomes and cell cortex and facilitate/maintain the association of Mud with Pins at the apical cortex. Our findings reveal that the centrosomal proteins Ana2 and Ctp regulate Mud function to orient the mitotic spindle during NB asymmetric division.
DOI	10.1016/j.devcel.2011.08.002
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Cell
Title	Developmental Cell
Publication Year	2001-
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Data from Reference
Aberrations (2)
	Df(1)JC70 Df(2R)Np3
Alleles (28)
	ana2¹⁶⁹ ana2⁷¹⁹ ana2^{C1.Scer\UAS.T:Avic\GFP-YFP.Venus} ana2^{C2.Scer\UAS.T:Avic\GFP-YFP.Venus} ana2^GD13865 ana2^{N1.Scer\UAS.T:Avic\GFP-YFP.Venus} ana2^{N2.Scer\UAS.T:Avic\GFP-YFP.Venus} ana2^{Scer\UAS.T:Avic\GFP-YFP.Venus} asl¹ asl^{FL.Ubi-p63E.T:Avic\GFP-EYFP} ctp^exc6 ctp^{Scer\UAS.T:Avic\GFP-YFP.Venus,T:Uuuu\CAAX} ctp^{Scer\UAS.T:Avic\GFP-YFP.Venus} Dhc64C^4-19 mud¹ mud^{C.Scer\UAS.T:Hsap\MYC} mud^{N.Scer\UAS.T:Hsap\MYC} mud^{ΔR.Scer\UAS.T:Hsap\MYC} mud^{ΔRT.Scer\UAS.T:Avic\GFP-YFP.Venus} mud^{ΔRT.Scer\UAS.T:Hsap\MYC} rap^{Ubi-p63E.T:Avic\GFP-m6} raps^P62 Sas-4^s2214 Scer\GAL4^ey-OK107 Scer\GAL4^insc-Mz1407 Scer\GAL4^wor.PA T:Avic\GFP^YFP.Venus αTub84B^{Ubi-p63E.T:Avic\GFP-S65C}
Constructs (17)
	P{GD13865} P{UAS-Myc-mud.C} P{UAS-Myc-mud.N} P{UAS-Myc-mud.ΔR} P{UAS-Myc-mud.ΔRT} P{UAS-Venus-ana2.C1} P{UAS-Venus-ana2.C2} P{UAS-Venus-ana2.N1} P{UAS-Venus-ana2.N2} P{UAS-Venus-ana2} P{UAS-Venus-ctp.CAAX} P{UAS-Venus-ctp} P{UAS-Venus-mud.ΔRT} P{Ubi-p63E-GFP-α-Tub84B} P{Ubi-p63E-rap.GFP} P{Ubi-YFP-asl.FL} P{wor.GAL4.A}
Genes (25)
	ana2 aPKC ase asl aur baz cnn ctp Dhc64C dpn insc mira msps mud numb pon rap raps Sas-4 Scer\GAL4 T:Hsap\MYC tacc αTub84B αTub84D γTub23C
Insertions (2)
	P{GawB}ey^OK107 P{GawB}insc^Mz1407
Natural transposons (1)
	P-element