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Citation
Bateman, J.R., Wu, C.T. (2008). A simple polymerase chain reaction-based method for the construction of recombinase-mediated cassette exchange donor vectors.  Genetics 180(3): 1763--1766.
FlyBase ID
FBrf0215615
Publication Type
Research paper
Abstract
Here we describe a simple method for generating donor vectors suitable for targeted transgenesis via recombinase-mediated cassette exchange (RMCE) using the PhiC31 integrase. This PCR-based strategy employs small attB "tails" on the primers used to amplify a sequence of interest, permitting the rapid creation of transgenes for in vivo analysis.
PubMed ID
PubMed Central ID
PMC2581973 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Genetics
    Title
    Genetics
    Publication Year
    1916-
    ISBN/ISSN
    0016-6731
    Data From Reference
    Natural transposons (1)
    Transgenic Constructs (2)