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Citation
Diegelmann, S., Bate, M., Landgraf, M. (2008). Gateway cloning vectors for the LexA-based binary expression system in Drosophila.  Fly 2(4): 236--239.
FlyBase ID
FBrf0215616
Publication Type
Research paper
Abstract

Studies on the formation of connections in the developing nervous system are greatly aided by methods that permit the differential visualisation and manipulation of pre- and postsynaptic partner neurons. This has been facilitated by the advent of the LexA-based, GAL4/UAS-independent, binary expression system. On the molecular side, the introduction of DNA sequences into expression vectors has been simplified by the Invitrogen Gateway cloning technology. We have developed cloning vectors that combine the Gateway cloning technology with the LexA-based genetic expression system. These vectors facilitate the creation of driver and reporter constructs for the generation of Drosophila transgenic lines for the new LexA-based binary transcriptional system. We further report a new LexA::GAD sensory neuron driver and a red fluorescent membrane targeted lexAop reporter designed to complement the existing GFP-based lexAop reporter. Using these transgenic lines we have been able to differentially label motor and sensory neuron projections in the ventral nerve cord of Drosophila larvae.

PubMed ID
PubMed Central ID
PMC2913122 (PMC) (EuropePMC)
DOI
Associated Information
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Fly
    Title
    Fly
    Publication Year
    2007-
    ISBN/ISSN
    1933-6934 1933-6942
    Data From Reference
    Alleles (5)
    Genes (6)
    Natural transposons (1)
    Insertions (1)
    Experimental Tools (4)
    Transgenic Constructs (4)